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Delivering SaCas9 mRNA by lentivirus-like bionanoparticles for transient expression and efficient genome editing.
Lu B, Javidi-Parsijani P, Makani V, Mehraein-Ghomi F, Sarhan WM, Sun D, Yoo KW, Atala ZP, Lyu P, Atala A
Nucleic Acids Res. 2019 Feb 13. pii: 5316732. doi: 10.1093/nar/gkz093.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
122944psPAX2-D64V-NC-MS2MS2 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an MS2 aptamer into lentivirus-like particles
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122945psPAX2-D64V-NC-PP7PP7 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an PP7 aptamer into lentivirus-like particles
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122946pSaCas9-1xms2-2x3’UTRFor expressing SaCas9 mRNA with one copy of MS2 aptamer and 2 copies of HBB 3' UTR
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122947pSaCas9-1xPP7-2x3'UTRFor expressing SaCas9 mRNA with one copy of PP7 aptamer and 2 copies of HBB 3' UTR
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