PurposeFor expressing SaCas9 mRNA with one copy of PP7 aptamer and 2 copies of HBB 3' UTR
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||122947||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameSaCas9-PP7-HBB 3' UTR
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSaCas9-1xPP7-2x3'UTR was a gift from Baisong Lu (Addgene plasmid # 122947 ; http://n2t.net/addgene:122947 ; RRID:Addgene_122947)
For your References section:Delivering SaCas9 mRNA by lentivirus-like bionanoparticles for transient expression and efficient genome editing. Lu B, Javidi-Parsijani P, Makani V, Mehraein-Ghomi F, Sarhan WM, Sun D, Yoo KW, Atala ZP, Lyu P, Atala A. Nucleic Acids Res. 2019 Feb 13. pii: 5316732. doi: 10.1093/nar/gkz093. 10.1093/nar/gkz093 PubMed 30759231