PurposeMS2 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an MS2 aptamer into lentivirus-like particles
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||122944||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameNC-MCP (MS2 coat protein) fusion protein in Gag
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Please note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:psPAX2-D64V-NC-MS2 was a gift from Baisong Lu (Addgene plasmid # 122944 ; http://n2t.net/addgene:122944 ; RRID:Addgene_122944)
For your References section:Delivering SaCas9 mRNA by lentivirus-like bionanoparticles for transient expression and efficient genome editing. Lu B, Javidi-Parsijani P, Makani V, Mehraein-Ghomi F, Sarhan WM, Sun D, Yoo KW, Atala ZP, Lyu P, Atala A. Nucleic Acids Res. 2019 Feb 13. pii: 5316732. doi: 10.1093/nar/gkz093. 10.1093/nar/gkz093 PubMed 30759231