A new generation of animal cell expression vectors based on the Semliki Forest virus replicon.
Liljestrom P, Garoff H
Biotechnology (N Y). 1991 Dec;9(12):1356-61. PubMed
Liljestrom P, Garoff H
Biotechnology (N Y). 1991 Dec;9(12):1356-61. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 92072 | pSFV3 | Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles. |
| 92073 | pSFV-Helper1 | This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles. |
| 92074 | pSFV3-lacZ | This is an expression plasmid for production of recombinant genomic SFV RNA encoding beta-galactosidase. |