165,971 results

1. pLenti-nSpCas9-NoABE

   Plasmid

   #209043

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. IFI16-FL-(1-729)

   Plasmid

   #35051

   Depositor

   Cheryl Arrowsmith

   Article

   Liao et al Structure. 2011 Mar 9;19(3):418-29.

   Insert

   IFI16 (IFI16 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 6, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pCAT206

   Plasmid

   #134883

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

   Plasmid

   #162877

   Purpose

   Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM

   Depositor

   Frederic Meunier

   Article

   Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

   Insert

   pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
   

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Insert consists of AA1-170

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-LC3

   Plasmid

   #21073

   Purpose

   Mammalian expression of rat LC3 fused to EGFP

   Depositor

   Tamotsu Yoshimori

   Article

   Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   First ATG is not included.

   Promoter

   CMV

   Available Since

   May 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ-CBE-SpRYn-Act3.0

   Plasmid

   #178958

   Purpose

   It consists of a SpRY nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2 -SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   hA3A-SpRY-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF.STAT3C.Ubc.GFP

   Plasmid

   #24983

   Purpose

   3rd generation lentiviral expression of constitutively active Stat3

   Depositor

   Linzhao Cheng

   Article

   Hillion et al Cancer Research 68, 10121, December 2008

   Insert

   STAT3 (STAT3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   A662C, N664C (constitutively active)

   Available Since

   May 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

   Plasmid

   #138151

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   hU6_Rfxcas13d-DR_BsmBI
   

   Use

   Lentiviral

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI WT

   Plasmid

   #207832

   Purpose

   telomere specific nuclease to create double-strand breaks at telomeres

   Depositor

   Roger Greenberg

   Article

   Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

   Insert

   TRF1 (TERF1 Human)
   

   Use

   Lentiviral

   Tags

   Flag, DD, ER, mCherry and FokI

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBB540

    Plasmid

    #27393

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    grpE, clpB
    

    Expression

    Bacterial

    Available Since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE

    Plasmid

    #176268

    Purpose

    Expression of dCas12f-VPR-mCherry in mammalian cells

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    dCas12f
    

    Use

    Lentiviral

    Tags

    HA-VPR-mCherry

    Expression

    Mammalian

    Mutation

    D326A, D510A

    Promoter

    PGK

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPomyces-2BD

    Plasmid

    #234428

    Purpose

    Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template

    Depositor

    Min-Kyu Oh

    Article

    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

    Insert

    Modified Cas9 with polyaspartate linking
    

    Expression

    Bacterial

    Mutation

    Linked polyaspartate using glycine-serine linker

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKD236-4b

    Plasmid

    #90956

    Purpose

    Expresses ThsS constitutively

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Insert

    ThsS
    

    Expression

    Bacterial

    Promoter

    Bba_J23104

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSH124-ssr

    Plasmid

    #198025

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    T0 terminator - Ap/Cb resistance cassette
    

    Expression

    Bacterial

    Promoter

    ApR promoter

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQC V5 MCS IRES Puro

    Plasmid

    #110342

    Purpose

    γ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Puromycin selection.

    Depositor

    Sandra Martha Gomes Dias

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. HPV-31neo

    Plasmid

    #153283

    Purpose

    Neomycin selectable HPV31 genome

    Depositor

    Elliot Androphy

    Article

    Gilson et al J Virol. 2020 Jul 30;94(16). pii: JVI.00

    Insert

    HPV-31 genome with Neo cassette
    

    Use

    Unspecified

    Mutation

    HindIII and CsiI site mutated from original Neo c…

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti CMV GFP DEST (736-1)

    Plasmid

    #19732

    Purpose

    3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expression

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi; Gateway destination vector

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRE-Tn7-143

    Plasmid

    #118559

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sYFP2
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBI-p53 R175H/EGFP

    Plasmid

    #16544

    Depositor

    Bert Vogelstein

    Article

    Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

    Insert

    p53 (TP53 Human)
    

    Expression

    Mammalian

    Mutation

    R175H

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. DsRed-rab11 DN

    Plasmid

    #12680

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB11A (Homo sapiens) (RAB11A Human)
    

    Tags

    DsRed and HA

    Expression

    Mammalian

    Mutation

    changed serine 25 to asparagine

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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