v5 ABE-eVLP
(Plasmid #228467)

• Purpose: Expresses MMLVgag(C507V)–ABE8e for producing v5 ABE-eVLPs
• Depositing Lab: David Liu
• Depositing Organization: Broad Institute
  Located in the United States of America
• Publication: Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.1038/s41587-024-02467-x.

Backbone

• Vector backbone: pCDNA3-like
• Total vector size (bp): 11365
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MMLVgag(C507V)–ABE8e
• Species: Synthetic
• Insert Size (bp): 6609
• Mutation: MMLVgag(C507V)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG

Cloning Information

• Cloning method: Other
• 5′ sequencing primer: T7 promoter

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pCMV-MMLVgag-3xNES-ABE8e (Plasmid #181751)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 228467-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 228467-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.