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pFLAG-HHARI
(Plasmid #17450)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17450 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 17450-D50 50 μg of DNA in Tris buffer $495
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DNA 17450-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pFlag-CMV2
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HHARI
  • Species
    H. sapiens (human)
  • Entrez Gene
    ARIH1 (a.k.a. ARI, HARI, HHARI, UBCH7BP)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Step1: HindIII-human HHARI (about 700bp, 5’ portion)-EcoRV fragment was made by PCR. Step2: Digestion of pCMV-sport6-HHAR1 with EcoR V + Not I, producing 5.3Kb, 1.2Kb fragments, take back 1.2 Kb fragment. Step3: Digestion of pFlagCMV2 with Hind III + Not I, taking back the vector 5.4 Kb. Step4: Ligation of 3 fragments.

Please note: Addgene NGS is not able to fully resolve the length of the poly-A tail in the 3'UTR.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLAG-HHARI was a gift from Dong-Er Zhang (Addgene plasmid # 17450 ; http://n2t.net/addgene:17450 ; RRID:Addgene_17450)

  • For your References section:

    ISG15 modification of the eIF4E cognate 4EHP enhances cap structure-binding activity of 4EHP. Okumura F, Zou W, Zhang DE. Genes Dev. 2007 Feb 1. 21(3):255-60. 10.1101/gad.1521607 PubMed 17289916
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