pGEX-4T HHARI RBR
(Plasmid #31254)

• Depositing Lab: Rachel Klevit
• Publication: Wenzel et al Nature. 2011 May 1. ():.

Backbone

• Vector backbone: pGEX-4T2
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Human Homologue of Ariadne (HHARI)
• Alt name: ARI-1
• Species: H. sapiens (human)
• Insert Size (bp): 660
• Mutation: RBR domain residues 177-395
• Entrez Gene: ARIH1 (a.k.a. ARI, HARI, HHARI, UBCH7BP)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: GST sequencing primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The lab of Katherine Borden
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Capili AD, Edghill EL, Wu, K and Borden KL (2004)Structure of the C-terminal RING Finger from a RING-IBR-RING/TRIAD Motif Reveals a Novel Zinc-binding Domain Distinct from a RING. J. Mol. Bio. 340:1117-29.