pGEX-4T2 Parkin RBR
(Plasmid #31255)

• Depositing Lab: Rachel Klevit
• Publication: Wenzel et al Nature. 2011 May 1. ():.

Backbone

• Vector backbone: pGEX-4T2
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Parkin
• Species: R. norvegicus (rat)
• Insert Size (bp): 750
• Mutation: RBR domain, residues 217-465
• Entrez Gene: Prkn (a.k.a. Park, Park2)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal1 (unknown if destroyed)
• 3′ cloning site: Not1 (unknown if destroyed)
• 5′ sequencing primer: GST sequencing primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The lab of Edward Fon
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Joch M. et al, (2007) Parkin-mediated monoubiquitination of the PDZ protein PICK1 regulates the activity of acid-sensing ion channels. Mol. Biol. Cell 18: 3105-18