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pGEX-parkin 77-465
(Plasmid #45970)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45970 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-6P-1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4966
  • Total vector size (bp) 6136
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Parkin 77-465
  • Alt name
    PARK2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1170
  • Mutation
    Codon usage optimized for E.coli expression. Deleted a.a. 1-76
  • Entrez Gene
    Prkn (a.k.a. Park, Park2)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer PGEXF
  • 3′ sequencing primer PGEXR
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jean-Francois Trempe performed PCR amplification from WT codon-optimized gene and subcloned it.
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-parkin 77-465 was a gift from Kalle Gehring (Addgene plasmid # 45970 ; http://n2t.net/addgene:45970 ; RRID:Addgene_45970)
  • For your References section:

    Structure of Parkin Reveals Mechanisms for Ubiquitin Ligase Activation. Trempe JF, Sauve V, Grenier K, Seirafi M, Tang MY, Menade M, Al-Abdul-Wahid S, Krett J, Wong K, Kozlov G, Nagar B, Fon EA, Gehring K. Science. 2013 May 9. 10.1126/science.1237908 PubMed 23661642