PurposeMammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74142||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5546
- Total vector size (bp) 7043
Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameFluorescent indicator for voltage imaging Red
Insert Size (bp)1497
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer TAATACGACTCACTATAGGG
- 3′ sequencing primer TAGAAGGCACAGTCGAGG (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CMV-FlicR1 was a gift from Robert Campbell (Addgene plasmid # 74142)
For your References section:A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices. Abdelfattah AS, Farhi SL, Zhao Y, Brinks D, Zou P, Ruangkittisakul A, Platisa J, Pieribone VA, Ballanyi K, Cohen AE, Campbell RE. J Neurosci. 2016 Feb 24;36(8):2458-72. doi: 10.1523/JNEUROSCI.3484-15.2016. 10.1523/JNEUROSCI.3484-15.2016 PubMed 26911693
Generated by Addgene from full sequence supplied by depositor.