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CMV Marina-T2A-nls-mCherry
(Plasmid #74216)


Item Catalog # Description Quantity Price (USD)
Plasmid 74216 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4900
  • Total vector size (bp) 6361
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    fluorescent protein voltage sensor
  • Alt name
    GEVI Marina
  • Species
    Ciona intestinalis
  • Insert Size (bp)
  • Mutation
    Ci-VSP contains R217Q mutation; super ecliptic pHluorin contains D147A, H148A, Y200V and A227D mutations. These mutations cause depolarization-dependent increase in fluorescence intensity. We replaced residues 71-91 within CiVSD with an amino acid sequence (KSRITSEGEYIPLDQIDINV) from the Kir2.1 channel Golgi-to-plasma membrane trafficking signal. The Kir 2.1 channel ER export signal sequence (FCYENEV) was added to C-terminus of super ecliptic pHluorin.
  • GenBank ID
    AB183035 AY533296
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site SphI (not destroyed)
  • 5′ sequencing primer gacgtaaatgggcggtaggcgtg
  • 3′ sequencing primer ccttgagcatctgacttctggcta
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

A portion of this plasmid was derived from a plasmid made by Dr. Atsushi Miyawaki, Laboratory for Cell Function Dynamics, Brain Science Institute, RIKEN, 2-1 Hirosawa, Saitama 351-0198, Japan.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV Marina-T2A-nls-mCherry was a gift from Vincent Pieribone (Addgene plasmid # 74216 ; ; RRID:Addgene_74216)
  • For your References section:

    Directed evolution of key residues in fluorescent protein inverses the polarity of voltage sensitivity in the genetically-encoded indicator ArcLight. Platisa J, Vasan G, Yang A, Pieribone VA. ACS Chem Neurosci. 2017 Jan 3. doi: 10.1021/acschemneuro.6b00234. 10.1021/acschemneuro.6b00234 PubMed 28045247