159,371 results

1. pC1-SypHer3s-IMS

   Plasmid

   #108120

   Purpose

   Mitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

   Insert

   SypHer3s-IMS
   

   Use

   Tags

   ratiometricYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP GR

   Plasmid

   #47504

   Purpose

   Expresses glucocorticoid receptor in mammalian cells

   Depositor

   Alice Wong

   Insert

   Glucocorticoid receptor (NR3C1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFPL-Gg

   Plasmid

   #61653

   Purpose

   Gateway vector for generating green fluorescent protein fusions in fungi

   Depositor

   Mark Farman

   Article

   Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

   Insert

   enhanced green fluorescent protein
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLCN DSB Repair Reporter (DRR)

   Plasmid

   #98895

   Purpose

   DSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   Neomycin-T2A-ISce1-ISce1-eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. MAC_N_KLF6

   Plasmid

   #167772

   Purpose

   Expresses KLF6 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   KLF6 (KLF6 Human)
   

   Use

   Tags

   MAC

   Expression

   Mammalian

   Mutation

   no mutations

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. GAPDH-CPG-TV

   Plasmid

   #70148

   Purpose

   a targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA

   Depositor

   Rudolf Jaenisch

   Article

   Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

   Insert

   5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh
   

   Use

   Mouse Targeting and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. SNM-Luc

   Plasmid

   #35156

   Depositor

   Linda Penn

   Article

   Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

   Insert

   Myc promoter (MYC Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Contains 502 bp of the myc promoter

   Promoter

   c-myc

   Available since

   Oct. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pTF-ahpC-rfp

   Plasmid

   #153035

   Purpose

   For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-rab7 WT

   Plasmid

   #12605

   Depositor

   Richard Pagano

   Article

   Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

   Insert

   RAB7 (Homo sapiens) (RAB7A Human)
   

   Use

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.2-hBIN1-V5

    Plasmid

    #27303

    Depositor

    Robin Shaw

    Article

    Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

    Insert

    bridging integrator 1 (BIN1), transcript variant 8 (BIN1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pTSAR3.5t

    Plasmid

    #210257

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    eBFP2
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTSAR3.4t

    Plasmid

    #210256

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    mCherry
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-CjABE8e

    Plasmid

    #189929

    Purpose

    Plasmid encoding CjABE8e driven by the CMV promoter for plasmid transfection.

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    CjABE8e
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    CjCas9 D8A

    Promoter

    CMV

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJ4M_hnRNPA2_FL_WT

    Plasmid

    #139109

    Purpose

    Express FL hnRNPA2 with a C-terminal MBP tag

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRIPZ

    Plasmid

    #206981

    Purpose

    empty pTRIPZ vector for cloning of doxycycline-inducible gene expression

    Depositor

    Ming Chen

    Article

    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCB1532H-RFP

    Plasmid

    #101855

    Purpose

    Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance with RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)

    Depositor

    Sophien Kamoun

    Article

    Pennington et al Figshare (2017)

    Type

    Empty backbone

    Use

    Fungal expression plasmid, tested in magnaporthe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-UbC-Tet1v4-dCas9

    Plasmid

    #232180

    Purpose

    Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

    Insert

    Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 S. pyogenes, Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    hUbC

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-FGFR2

    Plasmid

    #116741

    Purpose

    Lentiviral expression of FGFR2

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FGFR2 (FGFR2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac-Dual-M2

    Plasmid

    #135584

    Purpose

    High eukaryotic dual protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin of AcMNPV

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. evoBxb1_IVT

    Plasmid

    #222341

    Purpose

    Plasmid for evolved Bxb1 mRNA in vitro transcription template

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved Bxb1
    

    Use

    Template for in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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