163,606 results

1. pBT44c

   Plasmid

   #122600

   Purpose

   BE PACE selection, express C-intein-Cas9-ugi fusion

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   intein-dCas9-ugi fusion
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. CRISPR-StAR4GN (Lenti)

   Plasmid

   #222693

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PGK without BsmBI cutsite

   Promoter

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-GFP-NMHC II-B

   Plasmid

   #11348

   Depositor

   Robert Adelstein

   Article

   Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

   Insert

   NMHC II-B (MYH10 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs-c-Myc

   Plasmid

   #13375

   Purpose

   Retroviral expression of mouse c-Myc

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   myelocytomatosis oncogene (Myc Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX458-HypaCas9

   Plasmid

   #190604

   Purpose

   PX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Takahashi et al iScience. 2022 Nov 17;25(12):105619. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA Flag ERbeta

   Plasmid

   #35562

   Depositor

   Harish Srinivas

   Article

   Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

   Insert

   Estrogen Receptor beta (ESR2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223-CSNK1D

   Plasmid

   #23796

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   CSNK1D (CSNK1D Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pLVX-EF1a-CCO-IRES-Puromycin

   Plasmid

   #134861

   Purpose

   Expresses EGFP-tagged inner mitochondrial membrane marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   cytochrome c oxidase subunit VIII (COX8A Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKI_ΩBBMVP16&SRDXWUSm1

   Plasmid

   #220349

   Purpose

   Expresses BBM-VP16 and SRDX-WUSm1 in plant cells

   Depositor

   Tomoko Igawa

   Article

   Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.

   Inserts

   BABY BOOM (BBM Mustard Weed)
   WUSCHEL (WUS Mustard Weed)
   

   Use

   Tags

   Superman Repression Domain X (SRDX) and VP16 tran…

   Expression

   Plant

   Mutation

   two amino acid substitution in WUS-box: L255A L25…

   Promoter

   CaMV 35S and RPS5A

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-FLAG-Rheb-N153T

    Plasmid

    #19997

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    Ras homolog enriched in brain 1 - N153T (RHEB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed Asparagine 153 to Threonine

    Promoter

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pCMV5-ActRIIB

    Plasmid

    #13501

    Depositor

    Jeff Wrana

    Article

    Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

    Insert

    Activin receptor IIB (Acvr2b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. psPAX2-D64V-NC-MS2

    Plasmid

    #122944

    Purpose

    MS2 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an MS2 aptamer into lentivirus-like particles

    Depositor

    Baisong Lu

    Article

    Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

    Insert

    NC-MCP (MS2 coat protein) fusion protein in Gag
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D64V

    Promoter

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60226

    Purpose

    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Renilla luciferase
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

    Tags

    Cre-HA and Rluc-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available Since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDestTol2-QUAS:GFP-CAAX

    Plasmid

    #184813

    Purpose

    Used to generate the Tg(QUAS:GFP-CAAX)c591 transgenic line

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    QUAS:GFP-CAAX-pA
    

    Use

    Zebrafish tol2 transgenesis

    Tags

    GFP-CAAX

    Expression

    Mutation

    Promoter

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX304_CMV::mCherry-LgBiT-FLAG-NES

    Plasmid

    #199715

    Purpose

    Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

    Depositor

    Alice Ting

    Article

    Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

    Insert

    mCherry-LgBiT-FLAG-NES
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. T7_CC_Vpx-SIV_WPRE_IVT_Template

    Plasmid

    #216792

    Purpose

    Template for in vitro transcription of Vpx (SIV).

    Depositor

    Daniel Bauer

    Article

    Levesque et al Nat Biotechnol. 2024 May 28. doi: 10.103

    Insert

    Vpx (SIV)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. phage UbiC G3BP1-GFP-GFP

    Plasmid

    #119950

    Purpose

    Lentiviral vector expressing G3BP1-GFP fusion protein

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    UbiC

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCL1177

    Plasmid

    #217961

    Purpose

    Dual cistronic expression vector for the RAPID-release approach. H3.2-mCherry is released from mitochondria to the nucleus upon addition of rapamycin.

    Depositor

    Andrew Bowman

    Article

    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

    Insert

    Histone H3 (HIST2H3PS2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Prab-3::NLS::GFP

    Plasmid

    #68120

    Purpose

    Nuclear Localized GFP for pan-neuronal expression in C. Elegans

    Depositor

    Andrew Leifer

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

    Insert

    GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rab-3

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOpen-Tthlig

    Plasmid

    #165569

    Purpose

    Taq DNA Ligase

    Depositor

    FreeGenes Project, Drew Endy, Jennifer Molloy

    Insert

    Tth DNA Ligase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 25, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge