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pET21a-AspRS-His
Plasmid#124106PurposeBacterial expression plasmid for PURE system componentsDepositorInsertAspartate-tRNA ligase (aspS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TetOne-Puro-p62
Plasmid#204548PurposeExpresses p62 in a doxycycline-dependent manner in mammalian cellsDepositorInsertp62 (SQSTM1 Human)
UseRetroviralTagsExpressionMammalianMutationNonePromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAM18dRGA[MCS]
Plasmid#84683PurposeCan be used for the transformation of rickettsiaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 EGFP-3XNLS
Plasmid#58468PurposeExpresses a nuclear-targeted eGFP on a CMV promoterDepositorInsert3X NLS with 3X stop codons
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRE132
Plasmid#118486Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-mCherry-cNLS
Plasmid#138527PurposeInducible expression of mCherry with nuclear localization signalDepositorInsertmCherry
UseTags3x SV40 cNLSExpressionMammalianMutationPromoterTRE3GsAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-EGFP
Plasmid#27082PurposeIntegration-free (episomal) expression of EGFPDepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
human beta-catenin pcDNA3
Plasmid#16828PurposeMammalian expression of human beta-catenin with Flag tagDepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAD-GamS
Plasmid#99246PurposeExpresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.DepositorInsertgamS (with C-terminal 6xHis tag)
UseTags6xHis tagExpressionBacterialMutationPromoterT7lacAvailable sinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
p-Tet-O-Sox5-Puro
Plasmid#221667PurposeExpresses Sox5 in mammalian cellsDepositorInsertSox5 (Sox5 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
OPN3-DuET
Plasmid#213357PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertOPN3 (OPN3 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-GFP1-10
Plasmid#68715PurposeSplit GFP assayDepositorInsertGFP1-10
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.SGZ
Plasmid#196224PurposeFluorescent reporter for glutamate, third generation, variant 857 in SGZ backbone. iGluSnFR3.v857.SGZDepositorInsertpAAV hSyn FLP-FRT iGluSnFR3 v857.SGZ
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and Stargazin and PDZ dom…ExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDUAL CD81 (GFP)
Plasmid#86980PurposeLentiviral expression construct encoding CD81 and GFP from separate promotersDepositorInsertCD81 (CD81 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFV/hPGKAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pF155 pcDNA3.1(+)SOD1G93A
Plasmid#26401DepositorInsertSOD1 (SOD1 Human)
UseTagsExpressionMammalianMutationG93APromoterAvailable sinceNov. 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
PPRE-pNL1.3
Plasmid#84394PurposeSecreted Luciferase reporter for PPARgamma activityDepositorInsertPPARG response element (PPARG Human)
UseTagssecNlucExpressionMammalianMutationPromoterPPREAvailable sinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nb-gp41-GFP (MoonTag-Nb-GFP)
Plasmid#128602PurposeExpresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).DepositorInsertNanobody_gp41
UseLentiviralTagsGB1 and sfGFPExpressionMutationPromoterSFFVAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-CysRS-His
Plasmid#124107PurposeBacterial expression plasmid for PURE system componentsDepositorInsertCysteinyl-tRNA ligase (cysS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry
Plasmid#159655PurposeEmpty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only
