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pET-His6-ERK2-MEK1_R4F_coexpression Citations (7)

Originally described in: Reconstitution of mitogen-activated protein kinase phosphorylation cascades in bacteria. Efficient synthesis of active protein kinases.
Khokhlatchev A, Xu S, English J, Wu P, Schaefer E, Cobb MH J Biol Chem. 1997 Apr 25;272(17):11057-62.
PubMed Journal

Articles Citing pET-His6-ERK2-MEK1_R4F_coexpression

Articles
Structural Studies of ERK2 Protein Complexes. Weijman JF, Riedl SJ, Mace PD. Methods Mol Biol. 2017;1487:53-63. PubMed
Cofilin-1 phosphorylation catalyzed by ERK1/2 alters cardiac actin dynamics in dilated cardiomyopathy caused by lamin A/C gene mutation. Chatzifrangkeskou M, Yadin D, Marais T, Chardonnet S, Cohen-Tannoudji M, Mougenot N, Schmitt A, Crasto S, Di Pasquale E, Macquart C, Tanguy Y, Jebeniani I, Puceat M, Morales Rodriguez B, Goldmann WH, Dal Ferro M, Biferi MG, Knaus P, Bonne G, Worman HJ, Muchir A. Hum Mol Genet. 2018 Sep 1;27(17):3060-3078. doi: 10.1093/hmg/ddy215. PubMed
Tyr1 phosphorylation promotes phosphorylation of Ser2 on the C-terminal domain of eukaryotic RNA polymerase II by P-TEFb. Mayfield JE, Irani S, Escobar EE, Zhang Z, Burkholder NT, Robinson MR, Mehaffey MR, Sipe SN, Yang W, Prescott NA, Kathuria KR, Liu Z, Brodbelt JS, Zhang Y. Elife. 2019 Aug 6;8. pii: 48725. doi: 10.7554/eLife.48725. PubMed
Systematic Discovery of Short Linear Motifs Decodes Calcineurin Phosphatase Signaling. Wigington CP, Roy J, Damle NP, Yadav VK, Blikstad C, Resch E, Wong CJ, Mackay DR, Wang JT, Krystkowiak I, Bradburn DA, Tsekitsidou E, Hong SH, Kaderali MA, Xu SL, Stearns T, Gingras AC, Ullman KS, Ivarsson Y, Davey NE, Cyert MS. Mol Cell. 2020 Jul 16;79(2):342-358.e12. doi: 10.1016/j.molcel.2020.06.029. Epub 2020 Jul 8. PubMed
Bora phosphorylation substitutes in trans for T-loop phosphorylation in Aurora A to promote mitotic entry. Tavernier N, Thomas Y, Vigneron S, Maisonneuve P, Orlicky S, Mader P, Regmi SG, Van Hove L, Levinson NM, Gasmi-Seabrook G, Joly N, Poteau M, Velez-Aguilera G, Gavet O, Castro A, Dasso M, Lorca T, Sicheri F, Pintard L. Nat Commun. 2021 Mar 26;12(1):1899. doi: 10.1038/s41467-021-21922-w. PubMed
LanCLs add glutathione to dehydroamino acids generated at phosphorylated sites in the proteome. Lai KY, Galan SRG, Zeng Y, Zhou TH, He C, Raj R, Riedl J, Liu S, Chooi KP, Garg N, Zeng M, Jones LH, Hutchings GJ, Mohammed S, Nair SK, Chen J, Davis BG, van der Donk WA. Cell. 2021 Apr 27. pii: S0092-8674(21)00436-0. doi: 10.1016/j.cell.2021.04.001. PubMed

Associated Plasmids

MAP kinases differentially bind and phosphorylate NOS3 via two unique NOS3 sites. Solone XKV, Caldara AL, Wells B, Qiao H, Wade LR, Salerno JC, Helms KA, Smith KER, McMurry JL, Chrestensen CA. FEBS Open Bio. 2022 May;12(5):1075-1086. doi: 10.1002/2211-5463.13384. Epub 2022 Mar 15. PubMed

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