Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21275||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4800
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Gene/Insert nameAxin2 promoter
SpeciesM. musculus (mouse)
Insert Size (bp)5600
Entrez GeneAxin2 (a.k.a. A, Axi1, Axil, Condu, Conductin)
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
A 5.6-kb DNA fragment containing the Axin2 promoter, exon 1, intron 1, and part of exon 2 was subcloned from a mouse BAC clone and sequenced. In Author's map, exons are shown as gray boxes, and the sequence is numbered with bp 1 corresponding to the start of the cDNA sequence. Eight conserved Tcf/LEF
binding sites (underlined and designated T1 to T8) were identiﬁed.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Axin2-luciferase was a gift from Frank Costantini (Addgene plasmid # 21275 ; http://n2t.net/addgene:21275 ; RRID:Addgene_21275)
For your References section:Wnt/beta-catenin/Tcf signaling induces the transcription of Axin2, a negative regulator of the signaling pathway. Jho EH, Zhang T, Domon C, Joo CK, Freund JN, Costantini F. Mol Cell Biol. 2002 Feb . 22(4):1172-83. 10.1128/MCB.22.4.1172-1183.2002 PubMed 11809808
Map uploaded by the depositor.