|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21276||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerClontech (discontinued)
- Backbone size w/o insert (bp) 4300
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Gene/Insert nameAxin2 promoter
SpeciesM. musculus (mouse)
Insert Size (bp)5600
Entrez GeneAxin2 (a.k.a. A, Axi1, Axil, Condu, Conductin)
/ Fusion Protein
- d2EGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site HindIII / SmaI (destroyed during cloning)
- 5′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The 5.6-kb DNA fragment was excised from Axin2-Luciferase using KpnI and HindIII (the HindIII site was ﬁlled in with Klenow DNA polymerase) and cloned into the KpnI- and SmaI-digested pd2EGFP-1 vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Axin2-d2EGFP was a gift from Frank Costantini (Addgene plasmid # 21276 ; http://n2t.net/addgene:21276 ; RRID:Addgene_21276)
For your References section:Wnt/beta-catenin/Tcf signaling induces the transcription of Axin2, a negative regulator of the signaling pathway. Jho EH, Zhang T, Domon C, Joo CK, Freund JN, Costantini F. Mol Cell Biol. 2002 Feb . 22(4):1172-83. 10.1128/MCB.22.4.1172-1183.2002 PubMed 11809808