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pRK5F Smad3

Plasmid

#12625

Depositor

Rik Derynck

Article

Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only

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pSXneo 135(T2AG3)

Plasmid

#12402

Depositor

Titia de Lange

Article

Hanish et al Proc Natl Acad Sci U S A. 1994 Sep 13. 9

Insert

135(T2AG3)

Expression

Mammalian

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

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LL - hOCT4i -1

Plasmid

#12198

Depositor

George Daley

Article

Zaehres et al Stem Cells. 2005 Mar . 23(3):299-305.

Insert

OCT4 - RNAi (POU5F1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Sept. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro CXCR4 siRNA-1

Plasmid

#12271

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

CXCR4 siRNA (CXCR4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV5B-FLAG-SARA

Plasmid

#11738

Depositor

Jeff Wrana

Article

Tsukazaki et al Cell. 1998 Dec 11. 95(6):779-91.

Insert

SARA (ZFYVE9 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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1051 pBabe puroL Akt

Plasmid

#9011

Depositor

William Sellers

Article

DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

Insert

Akt1 (AKT1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
1272 pBabe puroL Myr HA Akt3

Plasmid

#9019

Depositor

William Sellers

Insert

Akt3 (AKT3 Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
1072 pBabe puroL myr Akt K179M T308A S473A

Plasmid

#9015

Depositor

William Sellers

Article

DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

Insert

Akt1 K179M T308A S473A (AKT1 Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

K179M T308A S473A

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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MLV Gag-RFP

Plasmid

#1814

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

Gag (gag MuLV)

Tags

RFP

Expression

Mammalian

Available Since

May 19, 2005

Availability

Academic Institutions and Nonprofits only

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pAAV2/2

Plasmid

#232199

Purpose

AAV packaging plasmid expressing AAV2 Rep and capsid proteins

Depositor

Melina Fan, Addgene Research Program

Insert

Rep2/Cap2

Use

AAV

Expression

Mammalian

Available Since

Sept. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3/mStayGold(c4)=UtrCH

Plasmid

#212020

Purpose

For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

UtrCH

Tags

mStayGold(c4)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_550

Plasmid

#203398

Purpose

All-in-one lentiviral expression of gRNA and EnAsCas12a

Depositor

John Doench

Article

Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

Insert

Enhanced Acidaminococcus Cas12a

Use

CRISPR and Lentiviral

Promoter

EF1a

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pCCL/IFNB1-d2eGFP-3’UTR

Plasmid

#180232

Purpose

Reporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Front Cell Infect Microbiol. 2022 Jan 18

Insert

d2eGFP

Use

Lentiviral

Promoter

1 kb upstream of IFNB1 CDS

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-PS9

Plasmid

#177944

Purpose

Transient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

eGFP (ORF1ab )

Expression

Mammalian

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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HyPer7.2DAAO-mito

Plasmid

#168304

Purpose

Chemogenetic fluorescent H2O2 reporter targeted to the mitochondria

Depositor

Thomas Michel

Article

Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

Insert

HyPer7.2DAAO-mito

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-TurboID(full)-HA-GPI

Plasmid

#166055

Purpose

Expresses the full length TurboID on the surface of astrocytes

Depositor

Scott Soderling

Article

Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

Insert

GPI anchored TurboID

Use

AAV

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.eGFP.2A.wtTau

Plasmid

#140424

Purpose

AAV.CBA.eGFP.2A.wtTau propagation reporter WT-2N4R-hTau

Depositor

Bradley Hyman

Article

Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

Insert

eGFP-2A-Tau (MAPT Synthetic, Human)

Use

AAV

Tags

eGFP

Promoter

CBA

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLARRY-EGFP

Plasmid

#140025

Purpose

Lentiviral backbone for cloning expressed barcodes in the 3'UTR of the EGFP mRNA

Depositor

Fernando Camargo

Article

Weinreb et al Science. 2020 Jan 23. pii: science.aaw33

Insert

EGFP

Use

Lentiviral

Promoter

EF1alpha

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.V1

Plasmid

#127847

Purpose

non-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

Insert

Synthetic construct isolate AAV-PHP.V1 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a mCherry

Plasmid

#129431

Purpose

expression of mCherry from EF1a promoter

Depositor

Oskar Laur

Insert

mCherry

Use

Lentiviral

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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