159,371 results

1. IKK-2 S177E S181E

   Plasmid

   #11105

   Purpose

   Mammalian expression of constitutively active human IKK-2

   Depositor

   Anjana Rao

   Article

   Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

   Insert

   IKK-2 (IKBKB Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S177E and S181E, constitutively active

   Promoter

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFC330

   Plasmid

   #87842

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection marker

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   pyrG
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and native pro…

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2attPC

   Plasmid

   #51547

   Purpose

   DICE donor plasmid without neural transcription factors.

   Depositor

   Michele Calos

   Article

   Zhu et al Nucleic Acids Res. 2013 Dec 4.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-TLR4-YFP

   Plasmid

   #13018

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 4 (TLR4 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. AiP12102 - pAAV-AiE0387m-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2102)

   Plasmid

   #208133

   Purpose

   Direct-expressing SYFP2 in astrocytes AAV Virus

   Depositor

   Boaz Levi, The Allen Institute for Brain Science

   Article

   Mich et al bioRxiv 2023.09.20.558718

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Beta Globin minimal promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLostfoX-Kan

   Plasmid

   #99865

   Purpose

   TfoX induction vector for chitin-pathway transformation (KanR)

   Depositor

   Mark Mandel

   Article

   Brooks JF et al Proc Natl Acad Sci U S A. 2014 Dec 2;111

   Insert

   tfoXVF
   

   Use

   V. fischeri suicide cloning vector, cmr, tcr

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-GFAP-EGFP

   Plasmid

   #50473

   Purpose

   EGFP under the control of GFAP promoter

   Depositor

   Bryan Roth

   Insert

   EGFP
   

   Use

   AAV

   Tags

   N/A

   Expression

   Mutation

   Promoter

   GFAP

   Available since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSEM318 - sgRNA mosTI unc-119 single-copy

   Plasmid

   #159822

   Purpose

   Single copy or array insertion by MosTI using split unc-119 selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI unc-119 single-copy
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX_lenti_osTir1_9Myc_P2A_Bsd

   Plasmid

   #139746

   Purpose

   lentiviral vector expressing rice E3 ligase Tir1 in mammalian cells with selection marker

   Depositor

   Robert Tjian

   Article

   Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

   Insert

   rice E3 ligase Tir1 (osTir1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKD3

    Plasmid

    #45604

    Depositor

    Barry L. Wanner

    Article

    Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

    Insert

    FRT-cat-FRT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    R6K

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. hCEP120-EGFP

    Plasmid

    #50382

    Purpose

    Over-expression of EGFP-tagged hCEP120

    Depositor

    Tang Tang

    Article

    Lin et al J Cell Biol. 2013 Jul 22;202(2):211-9. d

    Insert

    Human CEP120 (CEP120 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. AiP14801 - pAAV-AiE0390m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4801)

    Plasmid

    #208163

    Purpose

    AiE0390m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated Cre recombination in brain astrocytes

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Insert

    iCreR297T
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. FRE5

    Plasmid

    #62377

    Purpose

    Fluorescent reporter to quantify the ratio of two alternative splicing patterns.

    Depositor

    Thomas Cooper

    Article

    Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

    Insert

    dsRed and GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC-LYC

    Plasmid

    #53270

    Purpose

    Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

    Insert

    crtE, crtI, crtB
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. p-dCas9-JMJD2A-Hygro

    Plasmid

    #104410

    Purpose

    transient expression of dCas9-JMJD2A fusion protein

    Depositor

    Stephan Beck

    Insert

    JMJD2A (KDM4A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKOV

    Plasmid

    #25769

    Depositor

    George Church

    Article

    Link et al J Bacteriol. 1997 Oct . 179(20):6228-37.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. qTAG-N-Zeo-mStayGold

    Plasmid

    #227252

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mStayGold cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-Q23

    Plasmid

    #40261

    Depositor

    David Rubinsztein

    Article

    Narain et al J Med Genet. 1999 Oct;36(10):739-46.

    Insert

    HTT partial exon 1 Q23 (HTT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    expanded poly-Q

    Promoter

    CMV

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. PG13-mCherry

    Plasmid

    #195291

    Purpose

    mCherry reporter containing 13 copies of the p53-binding consensus sequence

    Depositor

    Hideyuki Oguro

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-R-diLACCO1

    Plasmid

    #208028

    Purpose

    Control biosensor for intracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-diLACCO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge