159,352 results

1. pCIP-AMPKa1_KD

   Plasmid

   #79011

   Purpose

   For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   AMPK alpha 1 (PRKAA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K47R

   Promoter

   pCAGGs

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKDsgRNA-rssA

   Plasmid

   #89957

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   rssA gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_PER1_WT

   Plasmid

   #82152

   Purpose

   Gateway Donor vector containing PER1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   PER1 (PER1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLR5-CAG-NLS-SNAP

   Plasmid

   #174879

   Purpose

   PiggyBac transposon vector for expression of NLS-SNAP

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   NLS-SNAP
   

   Use

   Piggybac vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-RAM-d2TTA::TRE-FLEX-MCS-WPRE-pA

   Plasmid

   #84467

   Purpose

   Empty pAAV-CRAM plasmid w. multiple cloning site (MCS)

   Depositor

   Yingxi Lin

   Article

   Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   pRAM and TRE

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMD.RVG.CVS24-N2c

   Plasmid

   #19712

   Depositor

   Manfred Schubert

   Article

   Mentis et al J Neurosci Methods. 2006 Oct 30. 157(2):

   Insert

   Rabies virus CVS24-N2c glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-Sp1

   Plasmid

   #12097

   Depositor

   Robert Tjian

   Insert

   Sp1 (SP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa1-87 deleted

   Promoter

   Available since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSicoR-eIF1a-GFP-RPL10a

   Plasmid

   #159890

   Purpose

   Expresses GFP-RPL10a for ribosomal immunoprecipitation

   Depositor

   James Ellis

   Article

   Rodrigues et al Cell Rep. 2020 Mar 24;30(12):4179-4196.e

   Insert

   RPL10a (RPL10A Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   eIF1a

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-myc3-CUL4A

   Plasmid

   #19951

   Depositor

   Yue Xiong

   Article

   Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

   Insert

   CUL4A (cullin 4A) (CUL4A Human)
   

   Use

   Tags

   myc3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVITRO-HPV18 L1L2

    Plasmid

    #52592

    Purpose

    Expresses HPV18 L1 and L2 in mammalian cells

    Depositor

    Richard Roden

    Article

    Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

    Inserts

    HPV18 L1
    HPV18 L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. GW1-Mito-pHRed

    Plasmid

    #31474

    Depositor

    Gary Yellen

    Article

    Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

    Insert

    Cox8-tagged pHRed
    

    Use

    Tags

    4xCox8

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-GRAB_r5-HT1.0

    Plasmid

    #208719

    Purpose

    Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Red fluorescent 5-HT sensor GRAB_r5-HT1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. MAC3-EGFR

    Plasmid

    #222610

    Purpose

    Expression of MAC3-tagged EGFR

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pBMN mEGFP

    Plasmid

    #188643

    Purpose

    This is the Lazarou lab's standard retrovirus vector backbone for expression of mEGFP-tag fusions onto the N-terminus of cloned genes

    Depositor

    Michael Lazarou

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac1-6His-DHX9

    Plasmid

    #211440

    Purpose

    generation of Bacmid for insect cell expression of DHX9 N-terminally fused to 6XHis-Tag

    Depositor

    Frank Grosse, Helmut Pospiech

    Article

    Zhang et al J Biol Chem. 1997 Apr 25. 272(17):11487-

    Insert

    DHX9 (DHX9 Human)
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHes7-Achilles-Hes7

    Plasmid

    #153528

    Purpose

    Expresses fusion protein of Achilles/YFP and mouse Hes7 from mouse Hes7 promoter. The reporter shows oscillatory expression with 2-3 periodicity when expressed in mouse presomitic mesoderm.

    Depositor

    Ryoichiro Kageyama

    Article

    Yoshioka-Kobayashi et al Nature. 2020 Apr;580(7801):119-123. doi:

    Insert

    Hes7 (Hes7 Mouse)
    

    Use

    Tags

    Achilles

    Expression

    Mammalian

    Mutation

    Promoter

    mouse Hes7 promoter

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTarget

    Plasmid

    #214052

    Purpose

    Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    Target sequence
    

    Use

    Target rna plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNon-target

    Plasmid

    #214053

    Purpose

    Expresses non-target sequence for Haliangium type III CRISPR-Cas complex; identical to pTarget (Addgene plasmid # 214052) but does not contain a protospacer.

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    Non-target sequence
    

    Use

    Non-target rna plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHochTypeIII

    Plasmid

    #214039

    Purpose

    Bacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    Cas10
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT

    Promoter

    T7 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-Rab7A

    Plasmid

    #61803

    Purpose

    Human Rab7A fluorescently tagged with GFP on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Tags

    2x HA and GFP

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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