165,764 results

1. eSpCas9(1.1)_NoFlag_hROSA26_Left

   Plasmid

   #191442

   Purpose

   Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   hROSA26 sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJT306 Kp03 Lenti pEF-attB LSR GFP

   Plasmid

   #184949

   Purpose

   Lentiviral delivery of recombinase landing pad

   Depositor

   Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Kp03 Lenti pEF1a-attB LSR GFP
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pG1AK-sfGFP

   Plasmid

   #71739

   Purpose

   Modular shuttle vector for Geobacillus and E. coli

   Depositor

   Tom Ellis

   Article

   Reeve et al ACS Synth Biol. 2016 Jul 1.

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Shuttle vector

   Expression

   Bacterial

   Promoter

   pRplsWT

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-tTA

   Plasmid

   #210501

   Purpose

   expresses LAUNCHER component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-tTA
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-p300

   Plasmid

   #23252

   Depositor

   Warner Greene

   Article

   Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

   Insert

   p300 (EP300 Human)
   

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF0024

   Plasmid

   #142528

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOXQ1 (FOXQ1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDRM98 mKate2 Blast

   Plasmid

   #174720

   Purpose

   Expresses the NLS-tagged mKate2 gene, P2A and the blasticidin resistance gene.

   Depositor

   Wayne Tilley

   Article

   Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

   Inserts

   mKate2
   BSD
   

   Use

   Lentiviral

   Tags

   P2A linker and SV40 NLS with GGS linker

   Expression

   Mammalian

   Promoter

   MND and MND (off mKate-P2A)

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_5x Gal4 UAS

   Plasmid

   #79119

   Purpose

   Base vector for cloning Gal4DBDVP64 inducible genes

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. MIGR1

   Plasmid

   #27490

   Depositor

   Warren Pear

   Article

   Pear et al Blood. 1998 Nov 15. 92(10):3780-92.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   GFP and IRES

   Expression

   Mammalian

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTubb3-MC

    Plasmid

    #87112

    Purpose

    minicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    GFP
    

    Use

    CRISPR

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSK9065

    Plasmid

    #84345

    Purpose

    tightly controlled expression of mRFP fusion proteins in S. aureus

    Depositor

    Neville Firth

    Article

    Brzoska et al Appl Environ Microbiol. 2013 May;79(9):3

    Type

    Empty backbone

    Tags

    mRFPmars

    Expression

    Bacterial

    Promoter

    Pxyl/tet

    Available Since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXs-GLIS1

    Plasmid

    #30166

    Purpose

    Retroviral expression of non-canonical human GLIS1

    Depositor

    Shinya Yamanaka

    Article

    Maekawa et al Nature. 2011 . 474(7350):225-9.

    Insert

    GLIS1 (GLIS1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIIIA/MS2-1

    Plasmid

    #220631

    Purpose

    For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.

    Depositor

    Marvin Wickens

    Article

    Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-6xGLY-His-tag-kanMX6

    Plasmid

    #20763

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Expression

    Yeast

    Available Since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. YE1-BE4max

    Plasmid

    #138155

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YE1)-nSpCas9-UGI-UGI
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R126E; within Cas9 D10A

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-T-rnVAPA-1-219

    Plasmid

    #13395

    Depositor

    Axel Brunger

    Article

    Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

    Insert

    VAP-A (Vapa Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Feb. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pG1C

    Plasmid

    #71740

    Purpose

    Modular shuttle vector for Geobacillus and E. coli

    Depositor

    Tom Ellis

    Article

    Reeve et al ACS Synth Biol. 2016 Jul 1.

    Type

    Empty backbone

    Use

    Synthetic Biology; Shuttle vector

    Expression

    Bacterial

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-CAG-jGCaMP8s-WPRE

    Plasmid

    #179256

    Purpose

    AAV-mediated expression of GCaMP8s under the CAG promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAVS1-shRNA

    Plasmid

    #82697

    Purpose

    For targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.

    Depositor

    Alan Mullen

    Article

    Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

    Type

    Empty backbone

    Use

    RNAi

    Promoter

    hPGK, U6

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-hSNCA-NE

    Plasmid

    #102361

    Purpose

    Overexpression of synuclein alpha in mammalian cells

    Depositor

    Shu Leong Ho

    Article

    Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

    Insert

    synuclein alpha (SNCA Human)
    

    Tags

    NE

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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