179,844 results
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Plasmid#85050PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGiantin (GOLGB1 Human)
ExpressionMammalianMutationaa 3131-3259PromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hGli1 flag3x
Plasmid#84922Purposemammalian expression of Gli1DepositorAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBMN(CMV-copGFP-Luc2-Puro)
Plasmid#80389PurposeTo make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection markerDepositorInsertcopGFP-T2A-Luc2-F2A-Puromycin
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-HA-YFP-rictor
Plasmid#73387PurposeExpresses HA and YFP tagged RictorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRPTOR Independent Companion Of MTOR, Complex 2 (RICTOR Human)
TagsHA and YFPExpressionMammalianPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-N-CretrcintG
Plasmid#69570PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cellsDepositorHas serviceAAV1Insertkozak consensus - N-CretrcintG
UseAAV and Synthetic BiologyExpressionMammalianPromoterEF1aAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
OPRM1-Tango
Plasmid#66464PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOPRM1 (OPRM1 Human)
TagsFLAG and tTAExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Str-Ii_VSVG-SBP-mCherry
Plasmid#65301Purposesynchronize trafficking of VSVG from the ER (RUSH system)DepositorInsertVSV-G
TagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEYFP-Sec24C
Plasmid#66608PurposeYFP fusion of Sec24CDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSec24C (SEC24C Human)
ExpressionMammalianAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-dCas9-FLp300
Plasmid#61356Purposeencodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertS.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Neo
Plasmid#60509PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and neomycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HA-RNF4/pRK5
Plasmid#59743PurposeExpresses RNF4 in mammalian cellsDepositorAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Nanog-2A-mCherry
Plasmid#59995PurposeInserts mCherry immediately downstream of the Nanog coding regionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNanog (Nanog Mouse)
UseMouse TargetingTags2A-mCherryAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-HA-hIFITM1
Plasmid#58399PurposeExpressed human IFITM1 with an N-terminal HA tag in mammalian cellsDepositorAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Calnexin-N-14
Plasmid#55005PurposeLocalization: Endoplasmic Reticulum, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCalnexin (CANX Human)
TagsmCherryExpressionMammalianMutationI474N in CalnexinPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5 FRT/TO SF-HERC2 (ShB-R)
Plasmid#55613PurposeDox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cellsDepositorInsert3xFLAG tagged HERC2 (WT) (HERC2 Human)
Tags3xFLAG and STREPExpressionMammalianMutationSilent mutations have been incorporated into the …Available sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pICSL11024 (pICH47732::NOSp-NPTII-OCST)
Plasmid#51144PurposeConfers KAN resistance in plantaDepositorInsertNOSp-NPTII-OCST
Available sinceFeb. 26, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV-PIG-Cre
Plasmid#50935Purposeretroviral expression of Cre recombinaseDepositorPublicationInsertCre recombinase
UseCre/Lox and RetroviralTagsNLSExpressionMammalianAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD1-dU6:1gRNA
Plasmid#49408Purposeexpression of gRNA under control of the Drosophila U6:1 promoterDepositorInsertdU6-1:gRNA
UseCRISPRExpressionInsectPromoterdU6-1Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUltra-Chili
Plasmid#48687Purpose3rd generation Lentiviral vector with bi-cistronic expression of dtomato and the gene of interest.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV10/3xflag-Clock
Plasmid#47334Purposefull length clock in pCMV10 with 3x flag at the N-terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertclock
Tags3x FlagExpressionMammalianPromoterCMVAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by