|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50935||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneMSCV PIG, Addgene plasmid # 18751
Backbone manufacturerScott Lowe
- Backbone size w/o insert (bp) 6300
Vector typeMammalian Expression, Retroviral, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameCre recombinase
Insert Size (bp)1000
/ Fusion Protein
- NLS (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer MSCV (Common Sequencing Primers)
Contains Puro resistance gene, IRES, and GFP downstream of PGK promoter.
The Nuclear Localization Sequence fusion Cre recombinase (NLS-Cre) was excised from pTZ-CreN vector (a gift from L. Nitschke).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MSCV-PIG-Cre was a gift from Pier Pandolfi (Addgene plasmid # 50935 ; http://n2t.net/addgene:50935 ; RRID:Addgene_50935)
For your References section:Crucial role of p53-dependent cellular senescence in suppression of Pten-deficient tumorigenesis. Chen Z, Trotman LC, Shaffer D, Lin HK, Dotan ZA, Niki M, Koutcher JA, Scher HI, Ludwig T, Gerald W, Cordon-Cardo C, Pandolfi PP. Nature. 2005 Aug 4;436(7051):725-30. 10.1038/nature03918 PubMed 16079851