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pET42b-HF-BE3
(Plasmid #87438)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 87438 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET_42b
  • Backbone size w/o insert (bp) 4983
  • Total vector size (bp) 10146
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HF-BE3
  • Alt name
    GGS-His6-rAPOBEC1-XTEN-nHF-Cas9-GGS-UGI-GGS-NLS
  • Species
    H. sapiens (human), R. norvegicus (rat); Bacillus phage PBS2
  • Insert Size (bp)
    5163
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET42b-HF-BE3 was a gift from David Liu (Addgene plasmid # 87438)
  • For your References section:

    Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery. Rees HA, Komor AC, Yeh WH, Caetano-Lopes J, Warman M, Edge ASB, Liu DR. Nat Commun. 2017 Jun 6;8:15790. doi: 10.1038/ncomms15790. 10.1038/ncomms15790 PubMed 28585549