165,750 results

1. pAAV-DIO-CAG-hM3Dq-P2A-dTomato

   Plasmid

   #202036

   Purpose

   AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM3Dq-2A-dTomato

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Insert

   hM3Dq
   

   Use

   AAV

   Tags

   dTomato

   Promoter

   CAG

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_AGIA-AirID

   Plasmid

   #182210

   Purpose

   Express AirID in mammalian cells.

   Depositor

   Sohei Ito, Shogo Nakano, Akira Nozawa, Tatsuya Sawasaki

   Article

   Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

   Insert

   AGIA-AirID
   

   Tags

   AGIA-tag

   Expression

   Mammalian

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-UPEmS-tevo

   Plasmid

   #228894

   Purpose

   Golden gate cloning (BsmBI) of pegRNAs with tevopreQ1 motif, and with constitutive RFP expression.

   Depositor

   Francisco J Sánchez-Rivera

   Article

   Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. 5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

   Plasmid

   #158652

   Purpose

   making transgenics expressing a UAS driven eGFP and NTR 2.0

   Depositor

   Jeff Mumm

   Article

   Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

   Insert

   5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
   

   Use

   Tol2-based transgenic expression in zebrafish/oth…

   Mutation

   F70A/F108Y

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDESTmycDDX17

   Plasmid

   #19876

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17 Human)
   

   Tags

   MYC

   Expression

   Mammalian

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223_MDM2_WT

   Plasmid

   #82897

   Purpose

   Gateway Donor vector containing MDM2, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   MDM2 (MDM2 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPomyces-2BD

   Plasmid

   #234428

   Purpose

   Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template

   Depositor

   Min-Kyu Oh

   Article

   Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

   Insert

   Modified Cas9 with polyaspartate linking
   

   Expression

   Bacterial

   Mutation

   Linked polyaspartate using glycine-serine linker

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. p36-p37-p38-p40-pET-Duet-1

   Plasmid

   #175049

   Purpose

   Overexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coli

   Depositor

   Brian Kelch

   Article

   Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   Replication factor C subunits 2-5 (RFC4, RFC2, RFC3, RFC5 Human)
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDF-1b-RFC140ΔN555

   Plasmid

   #175043

   Purpose

   Overexpression of human (Rfc1) p140ΔN555 in E.coli

   Depositor

   Brian Kelch

   Article

   Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   Replication factor C subunit 1 (RFC1 Human)
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cyto-roGFP

    Plasmid

    #49435

    Purpose

    Expresses thiol redox-sensitive ratiometric sensor roGFP in cytosol of mammalian cells

    Depositor

    Paul Schumacker

    Article

    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

    Insert

    roGFP2
    

    Use

    Adenoviral

    Expression

    Mammalian

    Mutation

    C48S, Q80R, S147C, and Q204C in Clontech's G…

    Promoter

    CMV

    Available Since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pInt_attP1_LCS_kanR

    Plasmid

    #205300

    Purpose

    Integrating plasmid for ORBIT in E. coli. Contains library cloning site (LCS), which has transcriptional terminators to insulate reporter constructs.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pATetO 6XHis-Taq DNA Ligase

    Plasmid

    #178798

    Purpose

    Inducible expression of Taq DNA Ligase in E. coli

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

    Insert

    Taq DNA Ligase
    

    Expression

    Bacterial

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti SpBsmBI sgRNA Hygro

    Plasmid

    #62205

    Purpose

    An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

    Depositor

    Rene Maehr

    Article

    Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTRIP-GFP-IRF3

    Plasmid

    #127663

    Purpose

    Over-express EGFP-IRF3

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    IRF3 (IRF3 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWXC078

    Plasmid

    #222961

    Purpose

    C. elegans codon-optimized mScarlet-I3 with 3 introns cloned into pPD95.75 backbone

    Depositor

    Oliver Hobert

    Article

    Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

    Insert

    mScarlet-I3
    

    Expression

    Worm

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-UAS-Luciferase

    Plasmid

    #104840

    Purpose

    SPARK reporter gene

    Depositor

    Alice Ting

    Article

    Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

    Insert

    Luciferase
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    UAS

    Available Since

    Jan. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-TEK-V5/HIS

    Plasmid

    #205611

    Purpose

    Expression of human TEK receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    TEK receptor tyrosine kinase (TEK Human)
    

    Tags

    V5-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF.STAT3C.Ubc.GFP

    Plasmid

    #24983

    Purpose

    3rd generation lentiviral expression of constitutively active Stat3

    Depositor

    Linzhao Cheng

    Article

    Hillion et al Cancer Research 68, 10121, December 2008

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    A662C, N664C (constitutively active)

    Available Since

    May 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLifeAct-mScarlet3_N1

    Plasmid

    #189767

    Purpose

    Construct for labelling actin filaments in mammalian cells with lifeact-mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    LifeAct-mScarlet3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. TurboID-mNeongreen-3xFLAG fusion pos3

    Plasmid

    #172864

    Purpose

    C. elegans codon-optimized gateway entry vector expressing TurboID-mNeongreen fusion protein

    Depositor

    Mario de Bono

    Article

    Artan et al J Biol Chem. 2021 Sep;297(3):101094. doi

    Insert

    TurboID
    

    Use

    Unspecified

    Tags

    FLAG, mNeongreen, and myc

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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