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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 11815 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5446
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCaspase 7
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SpeciesH. sapiens (human)
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Insert Size (bp)1000
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Entrez GeneCASP7 (a.k.a. CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3)
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Tag
/ Fusion Protein
- Flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-Casp7-Flag was a gift from Guy Salvesen (Addgene plasmid # 11815 ; http://n2t.net/addgene:11815 ; RRID:Addgene_11815) -
For your References section:
Biochemical characteristics of caspases-3, -6, -7, and -8. Stennicke HR, Salvesen GS. J Biol Chem. 1997 Oct 10. 272(41):25719-23. 10.1074/jbc.272.41.25719 PubMed 9325297