|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13263||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameLambda Red
Insert Size (bp)2000
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Original description can be found in Poteete & Fenton (1984) Virology, 134:161-167.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTP223 was a gift from Kenan Murphy (Addgene plasmid # 13263 ; http://n2t.net/addgene:13263 ; RRID:Addgene_13263)
For your References section:Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli. Murphy KC, Campellone KG. BMC Mol Biol. 2003 Dec 13. 4():11. 10.1186/1471-2199-4-11 PubMed 14672541