|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16182||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5005
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameApoptosis-inducing factor
SpeciesH. sapiens (human)
Insert Size (bp)1839
Entrez GeneAIFM1 (a.k.a. AIF, AUNX1, CMT2D, CMTX4, COWCK, COXPD6, DFNX5, NADMR, NAMSD, PDCD8, SEMDHL)
- Cloning method Gateway Cloning
- 5′ cloning site attP1 (destroyed during cloning)
- 3′ cloning site attP2 (destroyed during cloning)
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGTTCCGGTGTGGAGGCC-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG GTCTTCATGAATGTTGAATAGTT-3'.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pENTR-AIF was a gift from Huda Zoghbi (Addgene plasmid # 16182 ; http://n2t.net/addgene:16182 ; RRID:Addgene_16182)
For your References section:A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Lim J, Hao T, Shaw C, Patel AJ, Szabo G, Rual JF, Fisk CJ, Li N, Smolyar A, Hill DE, Barabasi AL, Vidal M, Zoghbi HY. Cell. 2006 May 19. 125(4):801-14. 10.1016/j.cell.2006.03.032 PubMed 16713569