|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45698||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBabe puro Gateway
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 7900
Modifications to backboneGateway cloning sequences flank region with gene insert for use with the Gateway system reagents.
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Gene/Insert nameFGFR2 IIIb
SpeciesH. sapiens (human)
Insert Size (bp)2400
Entrez GeneFGFR2 (a.k.a. BBDS, BEK, BFR-1, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25)
- Cloning method Gateway Cloning
- 5′ sequencing primer Gateway reagents
- 3′ sequencing primer Gateway reagents (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBp-FGFR2b-WT was a gift from Matthew Meyerson (Addgene plasmid # 45698 ; http://n2t.net/addgene:45698 ; RRID:Addgene_45698)
For your References section:Inhibitor-sensitive FGFR2 and FGFR3 mutations in lung squamous cell carcinoma. Liao RG, Jung J, Tchaicha JH, Wilkerson MD, Sivachenko A, Beauchamp EM, Liu Q, Pugh TJ, Pedamallu CS, Hayes DN, Gray NS, Getz G, Wong KK, Haddad RI, Meyerson M, Hammerman PS. Cancer Res. 2013 Jun 20. 10.1158/0008-5472.CAN-12-3950 PubMed 23786770