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mEmerald-VEGFR2-N
(Plasmid #54298)

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Item Catalog # Description Quantity Price (USD)
Plasmid 54298 Standard format: Plasmid sent in bacteria as agar stab 1 $94 *
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DNA 54298-D50 50 μg of DNA in Tris buffer $495 *
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DNA 54298-D100 100 μg of DNA in Tris buffer $585 *
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Backbone

  • Vector backbone
    mEmerald-N1
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VEGFR2
  • Alt name
    Kinase insert domain receptor
  • Alt name
    NM_002253.2
  • Species
    H. sapiens (human)
  • GenBank ID
  • Entrez Gene
    KDR (a.k.a. CD309, FLK1, VEGFR, VEGFR2)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEmerald (C terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note VEGFR2 contains a V297I mutation. This is a common gene variant.

Kinase insert domain receptor . Excitation = 487; Emission = 509. Localization data: Membrane Receptor

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mEmerald-VEGFR2-N was a gift from Michael Davidson (Addgene plasmid # 54298 ; http://n2t.net/addgene:54298 ; RRID:Addgene_54298)
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