163,606 results

1. pACEMam2-TRPML1

   Plasmid

   #238303

   Purpose

   Expression of human TRPML1 in mammalian cells

   Depositor

   Pravin Mahajan

   Article

   Judith et al Structure, 2025

   Insert

   TRPML1 (MCOLN1 Human)
   

   Use

   Tags

   8xHis-3xFLAG-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   hybrid CAG promoter

   Available Since

   June 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain

   Plasmid

   #228663

   Purpose

   Bacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Zika virus portion of NS2B protease coexpressed with NS3 protease
   

   Use

   Tags

   His-GST-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pLJC2-MTHFD1-3xFLAG

   Plasmid

   #217429

   Purpose

   Lentiviral overexpression of human MTHFD1

   Depositor

   Jason Cantor

   Article

   Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

   Insert

   MTHFD1 (MTHFD1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28:LanIII

   Plasmid

   #225075

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanIII (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanIII)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRE3G-human cGAS

   Plasmid

   #186853

   Purpose

   Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   Human cGAS without tags (Adamts1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   TRE3G

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28:LanIII-43

   Plasmid

   #225077

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanIII-43 (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanIII-43)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28:LanII

   Plasmid

   #225066

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanII (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanII)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDM068

   Plasmid

   #216811

   Purpose

   Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Type

   Empty backbone

   Use

   CRISPR; Fungal expression

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDM053

   Plasmid

   #216815

   Purpose

   Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for N-terminal tagging.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Insert

   mNeonGreen
   

   Use

   Tags

   RIPGLIN

   Expression

   Bacterial

   Mutation

   Aspergillus nidulans codon-adjusted

   Promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDM071

    Plasmid

    #216817

    Purpose

    Aspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for N-terminal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mCardinal
    

    Use

    Tags

    RIPGLIN

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLIK DNM1L-V5 neo

    Plasmid

    #220363

    Purpose

    Lentiviral expression vector for an inducible V5-tagged DNM1L construct

    Depositor

    Kevin Janes

    Insert

    DNM1L (DNM1L Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lenti-guide-puro mAtp6v1a-1

    Plasmid

    #198477

    Purpose

    lentiviral stable expression of mAtp6v1a gRNA 1

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mouse ATPv1a (Atp6v1a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-guide-puro mAtp6v1a-2

    Plasmid

    #198478

    Purpose

    lentiviral stable expression of mAtp6v1a gRNA 2

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mouse ATPv1a (Atp6v1a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. human KANK2 Ankyrin Repeats pET151

    Plasmid

    #164054

    Purpose

    Expresses human KANK2 Ank domain in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 2 (KANK2 Human)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Crimson/P2A-Control (pSLIK3)

    Plasmid

    #113858

    Purpose

    An isogenic synaptase-free control vector to evaluate recombination using endogenous functions for N-terminal Crimson/P2A fusion plasmids.

    Depositor

    Richard S Myers

    Article

    Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

    Insert

    E2-Crimson
    

    Use

    Lentiviral

    Tags

    P2A/Crimson

    Expression

    Mutation

    Promoter

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBBK88 pHomL-TDH3p-Neon(gfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)

    Plasmid

    #179072

    Purpose

    Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp)-Link-2xPH(PLCd), an overexpressed (bright) marker for the plasma membrane

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    TDH3p-Neon(gfp)-Link-2xPH(PLCd)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVW293

    Plasmid

    #139490

    Purpose

    Insertion of the pHSP12 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pHSP12 24xPP7sl
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    pHSP12

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pStA1BZ

    Plasmid

    #114164

    Purpose

    Start-Stop Assembly Level 1BZ plasmid

    Depositor

    John Heap

    Article

    Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pStA1BC

    Plasmid

    #114165

    Purpose

    Start-Stop Assembly Level 1BC plasmid

    Depositor

    John Heap

    Article

    Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPB_TRE3G_scFV-sfGFP

    Plasmid

    #235573

    Purpose

    Dox-inducible expression of control superfolder (sf)GFP fused with scFV

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    superfolder (sf)fGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge