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pRMC2

Plasmid

#68940

Purpose

tetracycline-inducible expression vector for Staphylococcus aureus

Depositor

Tim Foster

Article

Corrigan et al Plasmid. 2009 Mar;61(2):126-9. doi: 10.1

Type

Empty backbone

Use

Tet inducible e. coli/staphylococcus shuttle vect…

Expression

Bacterial

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-sgGFP-NT1

Plasmid

#46914

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1)

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

sgGFP-NT1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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ATeam1.03-nD/nA/pcDNA3

Plasmid

#51958

Purpose

mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

Depositor

Takeharu Nagai

Article

Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

Insert

ATeam1.03-nD/nA

Expression

Mammalian

Available Since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

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Lentiviral-TOP-dGFP-reporter

Plasmid

#14715

Purpose

Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

LEF-1/TCF-responsive promoter (Ctnnb1 )

Use

Lentiviral

Tags

d2 eGFP

Expression

Mammalian

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pAAVS1-tet-iCas9-BFP2

Plasmid

#125519

Purpose

all-in-one-inducible Cas9

Depositor

Jason Moffat

Article

Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

Insert

AAVS1-TET-ON 3G-SpCas9-P2A-BFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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mito-mStayGold_pTwistCMVBetaGlobWPRENeo

Plasmid

#221898

Purpose

Expresses mStayGold targeted to the mitochondria

Depositor

Janelia Integrative Imaging

Insert

cox8-mStayGold

Tags

Cox8

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCBC-MT1T2

Plasmid

#50593

Purpose

CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Insert

T1, gRNA scaffold, OsU3t plus, TaU3p, T2

Use

CRISPR; Pcr template

Expression

Plant

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pEGB 35S:Luciferase:Tnos (GB0110)

Plasmid

#68216

Purpose

Transcriptional unit for Firefly Luciferase Protein plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Luc

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-mEGFP

Plasmid

#175293

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with mEGFP

Tags

mEGFP

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2+-eGFP

Plasmid

#221710

Purpose

Expression of eGFP in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

eGFP

Use

Fish expression

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Pepper-fused sgRNA

Plasmid

#217520

Purpose

Expresses Pepper-fused sgRNA in mammalian cells with U6 promoter

Depositor

Xing Li

Article

Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

Insert

Pepper-fused sgRNA for spCas9

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCGN-ATF6 (1-373)

Plasmid

#27173

Purpose

For mammalian expression of the cytoplasmic domain of human ATF6with an HA tag.

Depositor

Ron Prywes

Article

Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

Insert

ATF6 (ATF6 Human)

Tags

HA

Expression

Mammalian

Mutation

Contains only aa 1-373 (please see depositor comm…

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-Tornado-nLuc

Plasmid

#212611

Purpose

For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

Tornado-CVB3-nLuc

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD/His-miRFP670

Plasmid

#79984

Purpose

Monomeric near-infrared fluorescent protein miRFP670 in bacterial plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP670

Expression

Bacterial

Promoter

araB

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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PiggyBac-TetO-Ascl1-neomycin

Plasmid

#176482

Purpose

TetO-Ascl1 in a PiggyBac vector with neomycin resistance

Depositor

Marius Wernig

Article

Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

Insert

Ascl1 (Ascl1 Mouse)

Expression

Mammalian

Promoter

TetO

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pWPI-N1ICD

Plasmid

#185525

Purpose

Constitutive overexpression of human NOTCH1 intracellular domain

Depositor

Sean Palecek, Eric Shusta

Article

Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

Insert

NOTCH1 intracellular domain (NOTCH1 Human)

Use

Lentiviral

Promoter

EF-1a

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.KIF13B

Plasmid

#224413

Purpose

Mammalian expression of Kif 13b

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

Kif 13b (Kif13b Mouse)

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis T5 Exonuclease

Plasmid

#206209

Purpose

Inducible expression of T5 exonuclease

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

Insert

T5 exonuclease

Expression

Bacterial

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) FMC-63 scFv-Fc (huFc)

Plasmid

#183252

Purpose

Soluble expression of the murine anti-human CD19 scFv-Fc (clone FMC-63). Human IgG1 Fc.

Depositor

Alexander McLellan

Insert

anti-CD19 scFvFc (FMC-63)

Expression

Mammalian

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV CEPIA3mt

Plasmid

#58219

Purpose

Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

CEPIA3mt

Tags

myc

Expression

Mammalian

Mutation

GCaMP2 N263Y E282V E334D M339L

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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