pRetrosuper Fbw7 shRNA-1
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15660||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6400
Vector typeMammalian Expression, Retroviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameFbw7 shRNA
SpeciesH. sapiens (human)
Insert Size (bp)64
Entrez GeneFBXW7 (a.k.a. AGO, CDC4, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII? (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer H1 (Common Sequencing Primers)
hairpin targets the sequence: ACAGGACAGTGTTTACAAA
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRetrosuper Fbw7 shRNA-1 was a gift from Martin Eilers (Addgene plasmid # 15660 ; http://n2t.net/addgene:15660 ; RRID:Addgene_15660)
For your References section:The ubiquitin-specific protease USP28 is required for MYC stability. Popov N, Wanzel M, Madiredjo M, Zhang D, Beijersbergen R, Bernards R, Moll R, Elledge SJ, Eilers M. Nat Cell Biol. 2007 Jul . 9(7):765-74. 10.1038/ncb1601 PubMed 17558397