|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20072||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5111
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1000
Entrez GenePOU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer pBABE 5 (Common Sequencing Primers)
Created by Maria J. Barrero.
cDNA was amplified from ESmRNA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMSCV-Flag-hOct4 was a gift from Juan Belmonte (Addgene plasmid # 20072 ; http://n2t.net/addgene:20072 ; RRID:Addgene_20072)
For your References section:Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Aasen T, Raya A, Barrero MJ, Garreta E, Consiglio A, Gonzalez F, Vassena R, Bilić J, Pekarik V, Tiscornia G, Edel M, Boué S, Belmonte JC. Nat Biotechnol. 2008 Nov . 26(11):1276-84. 10.1038/nbt.1503 PubMed 18931654