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pRosetta
(Plasmid #59700)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59700 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLKO.1
  • Total vector size (bp) 8765
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin, Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Puro
  • Tag / Fusion Protein
    • 2A (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    BlastR
  • Tag / Fusion Protein
    • 2A (C terminal on insert)

Gene/Insert 3

  • Gene/Insert name
    EGFP

Resource Information

Depositor Comments

This lentiviral vector can be used as a standard for titering experiments when trying to compare vectors with different selection markers. It can also be used to determine if selection conditions with blasticidin or puromycin are using too high or too low a concentration of drug. If there are surviving cells that are EGFP-negative, then drug concentration is too low. If there are dying cells that express EGFP, then drug concentration is likely too high.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRosetta was a gift from John Doench & David Root (Addgene plasmid # 59700 ; http://n2t.net/addgene:59700 ; RRID:Addgene_59700)