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157,564 results

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pROSA26-ERT2CreERT2

Plasmid

#149436

Purpose

ROSA26 targetting vector with tamoxifen-inducible Cre recombinase

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

ERT2-Cre-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

ERT2

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSEVA3b67Rb

Plasmid

#58311

Purpose

Shuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.

Depositor

Tom Ellis

Article

Wright et al ACS Synth Biol. 2014 May 13.

Insert

mRFP1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pBAD/His-B-iRFP

Plasmid

#31855

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Insert

iRFP

Use

Tags

Expression

Bacterial

Mutation

S13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D2…

Promoter

araBAD

Available since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pMJ204: pU6-SpuFz1_omegaRNA(350nt)-entry

Plasmid

#205268

Purpose

Human expression vector for SpuFz1 omegaRNA 5' +275 nt extended scaffold (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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STARR-seq luciferase validation vector_ORI_neg.cont

Plasmid

#99315

Purpose

Negative control for luciferase validation vectors

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Insert

neutral region; chr3: 55198740-55200041

Use

Luciferase; Starr-seq luciferase validation vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-SLC66A2_STOP

Plasmid

#221419

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC66A2 (PQLC1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDule2-Mb haloTyrRS C6

Plasmid

#160378

Purpose

C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.

Depositor

Ryan Mehl

Article

Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

Insert

C6 HaloTyrosine tRNA synthatase

Use

Synthetic Biology

Tags

Expression

Bacterial and Mammalian

Mutation

L270S, Y271L, N311G, C313T

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001149198)

Plasmid

#80248

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)

Plasmid

#79620

Purpose

Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Use

Tags

PmCDA1 and UGI

Expression

Mammalian

Mutation

D10A for nickase Cas9

Promoter

pCMV

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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wtBxb1

Plasmid

#222337

Purpose

Plasmid for expressing wild type Bxb1 in the mammalian cells

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized wild type Bxb1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1 CMYC

Plasmid

#164145

Purpose

Doxycycline inducible expression of c-MYC

Depositor

Richard Possemato

Insert

MYC (MYC Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Doxycycline inducible promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET16b-PCNA

Plasmid

#134898

Purpose

Bacterial expression and purification of human PCNA

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Insert

PCNA (PCNA Human)

Use

Tags

untagged

Expression

Bacterial

Mutation

fully synthetic, codon optimized for bacterial ex…

Promoter

T7

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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XLone-Puro Cas13d-eGFP U6 BbsI

Plasmid

#155184

Purpose

PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and U6 gRNA backbone

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Type

Empty backbone

Use

Unspecified; Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC34A2

Plasmid

#132088

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC34A2 (SLC34A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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psfGFP-LAMP1-mCherry (pHLARE)

Plasmid

#164477

Purpose

Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

Depositor

Diane Barber

Article

Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

Insert

LAMP1 (Lamp1 Rat)

Use

Tags

mCherry and superfolder GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TFORF1217

Plasmid

#142757

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PRRX1 (PRRX1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMGS7 (AAVS1sgRNA)

Plasmid

#126582

Purpose

A CRISPR/Cas9 construct to target the AAVS1 locus in humans

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

AAVS1 sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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B-gTEMP/pRSET-b

Plasmid

#188446

Purpose

B-gTEMP for bacterial expression

Depositor

Takeharu Nagai

Article

Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

Insert

B-gTEMP

Use

Tags

6xHis-tag

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209664

Purpose

Expression of iATPSnFR2, medium affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.HaloTag

Use

AAV

Tags

Expression

Mutation

A95K

Promoter

hSyn

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pPPED

Plasmid

#162468

Purpose

Destination Expression vector for dicot plant prime editing

Depositor

Adam Bogdanove

Article

Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

Inserts

Cas9H840A
attR gateway destination cassette

Use

Tags

MLV reverse transcriptase

Expression

Plant

Mutation

Change histidine 840 to Alanine based sequence o…

Promoter

2x35S

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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