165,685 results

1. pDESTmycDDX17

   Plasmid

   #19876

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17 Human)
   

   Tags

   MYC

   Expression

   Mammalian

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ABE9

   Plasmid

   #194208

   Purpose

   Mammalian expression of ABE9

   Depositor

   Dali Li

   Article

   Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

   Insert

   TadA-8e (N108Q/L145T)-nSpCas9
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGGS_NP_EBOV

   Plasmid

   #103049

   Purpose

   Encodes Ebola virus NP gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

   Depositor

   Elke Mühlberger

   Article

   Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

   Insert

   NP gene of Ebola virus (NP Zaire ebolavirus, Mayinga)
   

   Expression

   Mammalian

   Mutation

   silent mutation (T to C) at position 928 of NP ge…

   Promoter

   CAG

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCS2+-eGFP

   Plasmid

   #221710

   Purpose

   Expression of eGFP in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   eGFP
   

   Use

   Fish expression

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC57-LbNOX

   Plasmid

   #75285

   Purpose

   This plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

   Insert

   LbNOX
   

   Tags

   C-terminal FLAG tag with linker

   Mutation

   Human codon optimized

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti UBC ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)

   Plasmid

   #215012

   Purpose

   Express a ERphagy reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   KDEL
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. eGFP-KIF5B-Rigor

   Plasmid

   #172204

   Purpose

   Expresses KIF5B G234A rigor mutant tagged with eGFP in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

   Insert

   KIF5B (Kif5b Mouse)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTNT-B18R

   Plasmid

   #58978

   Purpose

   expresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Insert

   B18R
   

   Expression

   Bacterial

   Promoter

   T7/Sp6

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 1036 pcDNA3 Myr HA Akt1

   Plasmid

   #9008

   Purpose

   Mammalian expression of human Akt1 with myr, HA tags

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   Akt1 (AKT1 Human)
   

   Tags

   HA and myr

   Expression

   Mammalian

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR223_JAK2_WT

    Plasmid

    #81756

    Purpose

    Gateway Donor vector containing JAK2 , part of the Target Accelerator Plasmid Collection. *Please note this is not a true WT JAK2 sequence and contains a V617F mutation.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    JAK2 (JAK2 Human)
    

    Use

    Gateway entry vector

    Mutation

    V617F

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Red Glifon Nega

    Plasmid

    #163117

    Purpose

    Red fluorescent protein-based glucose negative control sensor (does not respond to glucose) for mammalian cells

    Depositor

    Takashi Tsuboi

    Article

    Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

    Insert

    Red Glifon Nega
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7

    Plasmid

    #175529

    Purpose

    Expression of HaloTag7 at the inner membrane of mitochondria in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Cox8a-HaloTag7
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLKO5.sgRNA.EFS.tRFP

    Plasmid

    #57823

    Purpose

    3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    tagRFP
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLVXT Sox4

    Plasmid

    #101121

    Purpose

    Sox4 coding region

    Depositor

    Joan Massague

    Article

    David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

    Insert

    SOX4 (Sox4 Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHN924

    Plasmid

    #178885

    Purpose

    Expression of GST-His8-HRV3C

    Depositor

    Kazuhiro Abe

    Insert

    human rhinovirus 3C protease
    

    Tags

    GST, His8

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MIGR1

    Plasmid

    #27490

    Depositor

    Warren Pear

    Article

    Pear et al Blood. 1998 Nov 15. 92(10):3780-92.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP and IRES

    Expression

    Mammalian

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-N1 Dynamin1 wt

    Plasmid

    #120313

    Purpose

    Mammalian expression plasmid of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUCmini-iCAP-AAV.MaCPNS2

    Plasmid

    #185137

    Purpose

    non-standard AAV2 rep-AAV-MaCPNS2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

    Insert

    Synthetic construct isolate AAV-MaCPNS2 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV_AncBE4max_P2A_GFP

    Plasmid

    #112100

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    AncBE4max_P2A_GFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLX307 Luciferase

    Plasmid

    #117734

    Purpose

    Open reading frame vector encoding Luciferase

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    firefly luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge