PurposeBacterial expression of a fluorescent protein, Blue Fluorescent Protein (BFP)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||91757||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonemodified pRSETB
- Backbone size w/o insert (bp) 2900
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsE. coli expressing T7 polymerase necessary for fluorescent protein production. Expresses fluorescent protein constitutively. Do not add IPTG.
Copy numberHigh Copy
Gene/Insert nameBlue Fluorescent Protein
SpeciesAequorea victoria (jellyfish)
Insert Size (bp)735
- Promoter T7
/ Fusion Protein
- Hexa-Histidine, Xpress epitope for detection with the anti-Xpress antibody, and enterokinase cleavage site. (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer T7 Promoter Forward
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids are used in University Molecular Biology Laboratories and by High School students to purify fluorescent proteins and create fluorescent protein plate artwork.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pNCS BFP was a gift from Erik Rodriguez & Roger Tsien (Addgene plasmid # 91757 ; http://n2t.net/addgene:91757 ; RRID:Addgene_91757)
For your References section:Understanding, improving and using green fluorescent proteins. Cubitt AB, Heim R, Adams SR, Boyd AE, Gross LA, Tsien RY. Trends Biochem Sci. 1995 Nov;20(11):448-55. S0968-0004(00)89099-4 [pii] PubMed 8578587