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(Plasmid #91757)


Item Catalog # Description Quantity Price (USD)
Plasmid 91757 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    modified pRSETB
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    E. coli expressing T7 polymerase necessary for fluorescent protein production. Expresses fluorescent protein constitutively. Do not add IPTG.
  • Copy number
    High Copy


  • Gene/Insert name
    Blue Fluorescent Protein
  • Alt name
  • Species
    Aequorea victoria (jellyfish)
  • Insert Size (bp)
  • Mutation
    GFP (Y66H)
  • Promoter T7
  • Tag / Fusion Protein
    • Hexa-Histidine, Xpress epitope for detection with the anti-Xpress antibody, and enterokinase cleavage site. (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer T7 Promoter Forward
  • 3′ sequencing primer T7 Terminator
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

These plasmids are used in University Molecular Biology Laboratories and by High School students to purify fluorescent proteins and create fluorescent protein plate artwork.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNCS BFP was a gift from Erik Rodriguez & Roger Tsien (Addgene plasmid # 91757 ; ; RRID:Addgene_91757)
  • For your References section:

    Understanding, improving and using green fluorescent proteins. Cubitt AB, Heim R, Adams SR, Boyd AE, Gross LA, Tsien RY. Trends Biochem Sci. 1995 Nov;20(11):448-55. S0968-0004(00)89099-4 [pii] PubMed 8578587