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pMT-Hrp48-ADARcd-V5

Plasmid

#81172

Purpose

identification of cell-specific targets of RNA binding proteins

Depositor

Michael Rosbash

Article

McMahon et al Cell. 2016 Mar 30. pii: S0092-8674(16)30

Insert

Hrp48-ADARcd fusion (Hrb27C Fly)

Expression

Insect

Promoter

Drosophila metallothionein promoter

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-YAPS127A

Plasmid

#166458

Purpose

Expresses fusion of mEGFP and YAPS127A

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A (YAP1 Human)

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPRa_all-in-one

Plasmid

#183695

Purpose

Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Type

Empty backbone

Use

CRISPR

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-TID-HA3-HIS3MX6

Plasmid

#227981

Purpose

To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hM4D(Gi)-mCherry

Plasmid

#50477

Purpose

Gi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoter

Depositor

Bryan Roth

Has Service

AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

hM4D(Gi)-mCherry (CHRM4 Human)

Use

AAV

Tags

mCherry

Mutation

See supplemental documents for DREADD mutations

Promoter

CaMKIIa

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pSIP403

Plasmid

#122028

Purpose

Plasmid for expression of gene of interest in Lactobacillus plantarum and L. sakei

Depositor

Lars Axelsson, Geir Mathiesen

Article

Sorvig et al Microbiology. 2005 Jul;151(Pt 7):2439-49

Insert

gusA

Expression

Bacterial

Promoter

sppA

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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LSL-Cas9-Rosa26TV

Plasmid

#61408

Purpose

Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

Cas9
EGFP

Use

CRISPR, Cre/Lox, and Mouse Targeting

Tags

3xFLAG and P2A

Expression

Mammalian

Mutation

human codon optimized

Promoter

CAG

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pXPR_016-sgATF4-3

Plasmid

#202456

Purpose

Expression of a guide RNA targeting ATF4

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

ATF4 gRNA (ATF4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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GST-RBD

Plasmid

#15247

Depositor

Martin Schwartz

Article

Ren et al EMBO J. 1999 Feb 1. 18(3):578-85.

Insert

Rhotekin (Rtkn Mouse)

Tags

GST

Expression

Bacterial

Mutation

amino acids 7-89 (Rho binding domain)

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro

Plasmid

#141391

Purpose

Lentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-N (N SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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PP43

Plasmid

#78953

Purpose

Figure 2. Expresses BxbI.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

BxbI

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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HIP-flash (HOP-flash mutant)

Plasmid

#83466

Purpose

Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)

Use

Luciferase

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pPMVAd3

Plasmid

#98298

Purpose

Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

Insert

PRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A

Expression

Yeast

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-IpgD

Plasmid

#183666

Purpose

N-terminally tagged Shigella flexneri IpgD for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pHTN_HaloTag_KIF5B[1-560]

Plasmid

#213403

Purpose

Truncated human kinesin-1 with HaloTag at the N-terminus

Depositor

Jonas Ries

Article

Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

Insert

HaloTag-KIF5B[1-560] (KIF5B Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ER-GCaMP3

Plasmid

#64854

Purpose

Endoplasmic reticulum (ER)-targeted version of the Ca2+ indicator GCaMP3; targeted to cytosolic face of ER membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CYP450-GCaMP3

Tags

N-terminal 27 amino acids from CYP450

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Tp-gfpmut3

Plasmid

#65033

Purpose

TpR mini-Tn7 vector, mobilizable, for GFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

gfpmut3

Expression

Bacterial

Available Since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-mTagBFP2

Plasmid

#122373

Purpose

Expresses mTagBFP2 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

mTagBFP2

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAKTaq

Plasmid

#25712

Purpose

Bacterial expression of Taq polymerase

Depositor

David Engelke

Article

Engelke et al Anal Biochem. 1990 Dec . 191(2):396-400.

Insert

Taq polymerase

Expression

Bacterial

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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p1322 HPV-16 E6

Plasmid

#8642

Depositor

Peter Howley

Article

Münger et al J Virol. 1989 Oct . 63(10):4417-21.

Insert

HPV-16 E6 (E6 Human papillomavirus)

Expression

Mammalian

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

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