161,256 results

1. Parp14-c012

   Plasmid

   #173099

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP14 (PARP14 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   W1003-A1190

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. EHD2-mCherry

   Plasmid

   #45933

   Purpose

   caveolar protein, confines caveolae to the plasma membrane

   Depositor

   Ari Helenius

   Article

   Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:

   Insert

   Eps-15 homology domain-containing protein2 (EHD2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3.1-V5-PTK2B

   Plasmid

   #127233

   Purpose

   Eukaryotic expression of PTK2B

   Depositor

   Kai Johnsson

   Article

   Haruki et al PLoS One. 2012;7(5):e37598. doi: 10.1371

   Insert

   PTK2B
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNBU2_erm_P1T_DP-GH023 - NanoLuc

   Plasmid

   #117727

   Purpose

   Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023 upstream of the NanoLuc reporter

   Depositor

   Andrew Goodman

   Article

   Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

   Insert

   NanoLuc
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCerulean3-N1

   Plasmid

   #54730

   Purpose

   Localization: N1 Cloning Vector, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Type

   Empty backbone

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a_T7-ARG1

   Plasmid

   #106473

   Purpose

   Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli

   Depositor

   Mikhail Shapiro

   Article

   Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

   Insert

   ARG1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Parp14-c010

   Plasmid

   #173100

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP14 (PARP14 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   F1208-G1388

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. minCMV-1xmNG-dPCP

   Plasmid

   #231092

   Purpose

   Encodes a 1xmNeonGreen-dPCP under control of a weak promoter (minCMV).

   Depositor

   John Ngo

   Insert

   1xmNG-dMCP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMinDis.X513-iNicSnFR3a-(CC93)-PM

   Plasmid

   #125122

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

   Depositor

   Henry Lester

   Article

   Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

   Insert

   iNicSnFR3a-(CC93)-PM
   

   Use

   Tags

   Ig-K-Chain and membrane anchoring sequence-PDGFR

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   T7

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCFJ420 - Peft-3::GFP::H2B (ubiquitous)

    Plasmid

    #34877

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    Peft-3 GFP H2B tbb-2 UTR
    

    Use

    Worm targeting

    Tags

    Expression

    Mutation

    S65C

    Promoter

    eef-1A.1 (eft-3)

    Available since

    Feb. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mRNA-CleanCap entry vector

    Plasmid

    #188249

    Purpose

    Cloning vector for generating template plasmids to transcribe mRNA, with mNeonGreen dropout following BsaI digestion

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNH-TrxT

    Plasmid

    #26106

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Jun 10. ():.

    Type

    Empty backbone

    Use

    Tags

    6xHis, TEV cleavage site, and TrxA

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28b_RanWT

    Plasmid

    #186270

    Purpose

    Expression vector of His-RanQ69L in E.coli

    Depositor

    Masahito Ikawa

    Article

    Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    GTP-binding nuclear protein Ran (Ran Mouse)
    

    Use

    Tags

    His tagged

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. LC001: pMVP/PB/Hygro-DEST

    Plasmid

    #121876

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ Hygro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PB-PEmax

    Plasmid

    #187893

    Purpose

    All-in-one prime editor piggyBac transposon with PEmax

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    BPSV40 NLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR myc-MBP-SspB-iRFP-NLS

    Plasmid

    #223286

    Purpose

    An SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system.

    Depositor

    Jared Toettcher

    Article

    Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

    Insert

    Myc-MBP-SspB-irFP-NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-HA-RIPK1

    Plasmid

    #78834

    Purpose

    To overexpress RIPK1 in Mammalian Cell

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. IDG_SCN2B_OE_1

    Plasmid

    #161624

    Purpose

    Overexpresses SCN2B

    Depositor

    Mike McManus

    Insert

    SCN2B (SCN2B Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1_ADAR2DD_E488Q

    Plasmid

    #205598

    Purpose

    expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) in mammalian cells

    Depositor

    Marc Friedlander

    Article

    Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

    Insert

    ADAR2 deaminase domain (ADARB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E488Q hyperactive mutation

    Promoter

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS-mTagBFP2-T2A-iCre (v2)

    Plasmid

    #125822

    Purpose

    Monomeric TagBFP2 and iCre recombinase (version 2)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge