165,343 results

1. R777-E004 Hs.AKT2-nostop

   Plasmid

   #70288

   Purpose

   Gateway ORF clone of human AKT2 [NM_001626.5] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   AKT 2 (AKT2 Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-C1-ADRP

   Plasmid

   #87161

   Purpose

   Fluorescent protein localized to lipid droplets (LDs)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   ADRP (PLIN2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-N BRRF2

   Plasmid

   #37944

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   BRRF2 (BRRF2 H. Herpesvirus 4 (EBV))
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   PKG

   Available Since

   Aug. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

4. HER2Nb-C AND (A OR T)-SpyTag

   Plasmid

   #243790

   Purpose

   Expresses multifunctional construct enabling the AND(OR)-gated release of a HER2 Nanobody from materials in response to HRV-3C AND (sortase eSrtA(2A9) OR TEV) treatment.

   Depositor

   Cole DeForest

   Article

   Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

   Insert

   HER2 nanobody
   

   Tags

   6x His tag and SsrA

   Expression

   Bacterial

   Available Since

   Oct. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. TVMV-TEV-HRV-3C Proteases-mCherry

   Plasmid

   #243798

   Purpose

   Encodes for mammalian expression of TVMV, TEV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

   Depositor

   Cole DeForest

   Article

   Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

   Insert

   TVMV, TEV and HRV-3C proteases
   

   Expression

   Mammalian

   Available Since

   Oct. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUW-TetO-RORC

   Plasmid

   #207198

   Purpose

   doxycycline-inducible expression of Human RORC in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

   Insert

   pFUW-TetO-RORC
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVXCMV100-mNeonGreen-PBD

   Plasmid

   #241366

   Purpose

   Lentiviral expression of mNeonGreen-tagged PAK1 p21-binding domain (PBD)

   Depositor

   Gaudenz Danuser

   Article

   Weiss et al Sci Signal. 2025 May 20;18(887):eadk0922

   Insert

   Pak1 fragment (PAK1 Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Mutation

   aa 57-141

   Promoter

   Truncated CMV promoter

   Available Since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV gfaABC1D:NES-jRGECO1a

   Plasmid

   #239682

   Purpose

   Red calcium indicator jRGECO1a with a nuclear export signal expressed under an astrocyte-specific promoter.

   Depositor

   Marianne Fyhn

   Insert

   jRGECO1a
   

   Use

   AAV

   Tags

   NES

   Expression

   Mammalian

   Promoter

   gfaABC1D

   Available Since

   July 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKG1334_pHarbor-PiggyBac_backbone

   Plasmid

   #239705

   Purpose

   PaqCI Golden Gate assembly plasmid encoding the backbone for PiggyBac-mediated integration

   Depositor

   Kate Galloway

   Article

   Peterman et al Nucleic Acids Research, Volume 53, Issue

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. HCT55_rAAV-eHGT453m_eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA

    Plasmid

    #223945

    Purpose

    Direct-expressing SYFP2 AAV virus

    Depositor

    Allen Institute for Brain Science, Tanya Daigle

    Article

    Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

    Insert

    SYFP2
    

    Use

    AAV

    Mutation

    N/A

    Promoter

    minBglobin

    Available Since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-HaloCKAR2.2-T2A-EGFP

    Plasmid

    #216493

    Purpose

    Expression of chemigenetic PKC biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloCKAR2.2
    

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-HaloAKAR2.1-CLC

    Plasmid

    #216492

    Purpose

    Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) targeted to clathrin coated pits

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.1-CLC
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRetro-CAG-DsRedExpress-TVA

    Plasmid

    #235699

    Purpose

    Control plasmid expressing TVA and DsRed without G protein

    Depositor

    Hongjun Song, Guo-li Ming

    Article

    Sun et al Nature. 2025 Jan 16. doi: 10.1038/s41586

    Insert

    DsRedExpress, TVA800
    

    Use

    Retroviral

    Promoter

    CAG

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRetro-CAG-DsRedExpress-G-TVA

    Plasmid

    #235698

    Purpose

    Expresses G protein, TVA receptor, and DsRed for rabies virus trans-synaptic tracing

    Depositor

    Hongjun Song, Guo-li Ming

    Article

    Sun et al Nature. 2025 Jan 16. doi: 10.1038/s41586

    Insert

    DsRedExpress, TVA800, and Glyco
    

    Use

    Retroviral

    Promoter

    CAG

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR_CMV_MeltTEVp-40-mCherry

    Plasmid

    #234064

    Purpose

    Encodes a TEV protease controlled by a Melt variant with a switching temperature of 40°C

    Depositor

    Lukasz Bugaj

    Article

    Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

    Insert

    MeltTEV-40-mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFAP-Flex-MCS-WPRE-SV40 - GCH32

    Plasmid

    #230830

    Purpose

    AAV cloning vector for CRE-dependent expression under the GFAP promoter.

    Depositor

    Kimberly Ritola

    Type

    Empty backbone

    Use

    AAV and Cre/Lox; Cloning vector

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSR1154

    Plasmid

    #221653

    Purpose

    Tetracycline sensor v3 for Staphylococcus aureus

    Depositor

    Lauren Andrews

    Article

    Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

    Inserts

    sfGFP cassette
    TetR cassette
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PXyl-TetO and PZwf

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5/FRT-hMAPT

    Plasmid

    #231583

    Purpose

    Minigene of human MAPT containing exon 9, 10, 11 and relevant intronic sequences

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Cell Genom. 2024 Jun 12;4(6):100563. doi

    Insert

    human MAPT (MAPT Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR223-BPLF1_2001-3000

    Plasmid

    #198586

    Purpose

    Gateway Entry Clone

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BPLF1_2001-3000
    

    Use

    Gateway entry vector

    Mutation

    None (wildtype)

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF0251

    Plasmid

    #142574

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    VEZF1 (VEZF1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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