Spatial regulation of AMPK signaling revealed by a sensitive kinase activity reporter.
Schmitt DL, Curtis SD, Lyons AC, Zhang JF, Chen M, He CY, Mehta S, Shaw RJ, Zhang J
Nat Commun. 2022 Jul 5;13(1):3856. doi: 10.1038/s41467-022-31190-x. PubMed Article
Schmitt DL, Curtis SD, Lyons AC, Zhang JF, Chen M, He CY, Mehta S, Shaw RJ, Zhang J
Nat Commun. 2022 Jul 5;13(1):3856. doi: 10.1038/s41467-022-31190-x. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192446 | pcDNA3-ExRai-AMPKAR | Genetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells. |
| 192447 | pcDNA3-ExRai-AMPKAR(T/A) | Non-phosphorylatable negative-control mutant of ExRai-AMPKAR. |
| 192448 | pcDNA3-Mito-ExRai-AMPKAR | ExRai-AMPKAR targeted to the mitochondrial outer membrane. |
| 192449 | pcDNA3-Lyso-ExRai-AMPKAR | ExRai-AMPKAR targeted to the lysosomal membrane. |
| 192450 | pcDNA3-Nuc-ExRai-AMPKAR | ExRai-AMPKAR targeted to the nucleus. |
| 192451 | pEGFP-C1-mScarlet-AMPKα2 | mScarlet-tagged alpha2 isoform of AMPK catalytic subunit. |
| 192452 | pEGFP-C1-3x-NLS-mScarlet-AMPKα2 | mScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus. |