|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18030||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6620
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Growth instructionsDH5a, HB101
Copy numberLow Copy
SpeciesM. musculus (mouse)
Insert Size (bp)1300
Entrez GeneWnt3a (a.k.a. Wnt-, Wnt-3a, vt)
/ Fusion Protein
- HA (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer AGCTCGTTTAGTGAACCGTCAGATCG
- 3′ sequencing primer ACCTACAGGTGGGGTCTTTCATTCCC (Common Sequencing Primers)
Gavin, B.J., McMahon, J.A., and McMahon, A.P. Expression of multiple novel Wnt-1/Int-1-related genes during fetal and adult mouse development. Genes Dev.,4:2319-2332, 1990.
Roelink, H., and Nusse, R. Expression of two members of the Wnt family during mouse development -restricted temporal and spatial patterns in the developing neural tube. Genes Dev., 5: 381-388, 1991.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLNC Wnt-3aHA was a gift from Jan Kitajewski (Addgene plasmid # 18030 ; http://n2t.net/addgene:18030 ; RRID:Addgene_18030)
For your References section:Transformation by Wnt family proteins correlates with regulation of beta-catenin. Shimizu H, Julius MA, Giarre M, Zheng Z, Brown AM, Kitajewski J. Cell Growth Differ. 1997 Dec . 8(12):1349-58. PubMed 9419423