|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||99248||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerBock lab
- Backbone size (bp) 10214
Modifications to backboneremoved replaced EF1a promoter with EFS promoter. Removed Puromycin resistant gene and added SpCas9-P2A-EGFP
Vector typeMammalian Expression, Lentiviral, CRISPR
- Promoter EFS
/ Fusion Protein
- EGFP (C terminal on backbone)
Growth in Bacteria
Growth Strain(s)NEB Stable
- Cloning method Gibson Cloning
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CROPseq-Guide-EFS-SpCas9-P2A-EGFP was a gift from Alex Hewitt (Addgene plasmid # 99248 ; http://n2t.net/addgene:99248 ; RRID:Addgene_99248)