162,717 results

1. pUC19-TgKI-MCS-p2A-mScarlet-MCS-polyA

   Plasmid

   #174029

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   p2A-mScarlet
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ131A2.0

   Plasmid

   #99884

   Purpose

   Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRIS-PITChv2-BSD-dTAG (BRD4)

   Plasmid

   #91792

   Purpose

   PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   BSD-P2A-2xHA-FKBP_F36V
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promoterless

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-SpCas9-NG

   Plasmid

   #117919

   Purpose

   Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

   Depositor

   Osamu Nureki

   Article

   Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

   Insert

   SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Neo-Bam p53 R175H

   Plasmid

   #16436

   Depositor

   Bert Vogelstein

   Article

   Baker et al Science. 1990 Aug 24. 249(4971):912-5.

   Insert

   p53 R175H (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R175H

   Promoter

   CMV

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-tmpknot-GG-acceptor

   Plasmid

   #174039

   Purpose

   Prime editing in mammalian cells

   Depositor

   David Liu

   Article

   Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEAK10-His-Myc-Kal12

   Plasmid

   #25442

   Depositor

   Betty Eipper

   Article

   Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

   Insert

   Kal12
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pYPQ132A2.0

   Plasmid

   #99887

   Purpose

   Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR_PGK_SNIPR_CD8alpha variant 4

   Plasmid

   #188364

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant4-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant4-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ133A2.0

    Plasmid

    #99891

    Purpose

    Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKM-U6-reRNA-acceptor-2xBbsI

    Plasmid

    #205633

    Purpose

    Cloning and U6-drive expression of ISDra2 TnpB reRNAs

    Depositor

    Gerald Schwank

    Article

    Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

    Insert

    ISDra2-TnpB reRNA with placeholder for guide cloning with BbsI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-hDAT

    Plasmid

    #32810

    Depositor

    Susan Amara

    Insert

    DAT (SLC6A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB-mTSPAN4-mCherry

    Plasmid

    #210330

    Purpose

    Overexpression of mTSPAN4

    Depositor

    Li Yu

    Article

    Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

    Insert

    mTSPAN4 (Tspan4 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR_PGK_SNIPR_CD8alpha variant 2, G318A

    Plasmid

    #188365

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD_G318A, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_G318A_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCPmacroH2A.1-GS3

    Plasmid

    #204759

    Purpose

    Expression of Cas9 and human macroH2A.1

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    macroH2A1 (MACROH2A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA6A-EGFR WT

    Plasmid

    #42665

    Depositor

    Mien-Chie Hung

    Article

    Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pR BirA

    Plasmid

    #113640

    Purpose

    Insect cell expression vector for BirA Biotin Ligase

    Depositor

    James Moon

    Article

    Moon et al Methods Mol Biol. 2018;1799:165-181. doi

    Insert

    BirA Biotin Ligase (birA )
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. cacna2d1 pCAGGS

    Plasmid

    #206104

    Purpose

    expression of rat alpha2delta-1 calcium channel auxillary subunit

    Depositor

    Annette Dolphin

    Insert

    cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/B-actin

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLPCX roGFP2-Orp1

    Plasmid

    #64991

    Purpose

    Mammalian expression of cytosolic roGFP2-Orp1 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTSin EGFP

    Plasmid

    #127695

    Purpose

    Plasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequence

    Depositor

    Bryan Roth, Justin English

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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