161,256 results

1. pLJC5-LAMP1-RFP-2xFlag

   Plasmid

   #102931

   Purpose

   Express a lysosomal Tag

   Depositor

   David Sabatini

   Article

   Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

   Insert

   LAMP1
   

   Use

   Lentiviral

   Tags

   mRFP-2xFlag

   Expression

   Mutation

   Promoter

   ubc

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. GST PTBP1 isoform 4

   Plasmid

   #108594

   Purpose

   Recombinant GST tagged protein production

   Depositor

   Chris Smith

   Article

   Coelho et al EMBO J. 2015 Mar 4;34(5):653-68. doi: 10

   Insert

   PTBP1 isoform 4 (PTBP1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   isoform 4 (isoform a)

   Promoter

   tac

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUW-OSKM

   Plasmid

   #20328

   Purpose

   Lentiviral plasmid expressing mouse Oct4, Sox2, Klf4 and cMyc for iPS cell generation

   Depositor

   Rudolf Jaenisch

   Article

   Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

   Insert

   Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Myc, Sox2, Pou5f1, Klf4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRIPZ shIFNAR

   Plasmid

   #127699

   Purpose

   Doxycyclin inducible shRNA knockdown of mouse IFNAR gene

   Depositor

   Sandra Demaria

   Article

   Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

   Insert

   shIFNAR1 Mmu (Ifnar1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJH-MBP-CbAgo

   Plasmid

   #127707

   Purpose

   pET His6 MBP TEV CbAgo

   Depositor

   John van der Oost

   Article

   Hegge et al Nucleic Acids Res. 2019 May 9. pii: 5487

   Insert

   CbAgo
   

   Use

   Tags

   MPB

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJCSUP35

   Plasmid

   #1088

   Depositor

   Susan Lindquist

   Article

   Glover et al Cell 1997 May 30;89(5):811-9.

   Insert

   SUP35 (SUP35 Budding Yeast)
   

   Use

   Tags

   HIS tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 26, 2005

   Availability

   Academic Institutions and Nonprofits only

7. PARP9-c020

   Plasmid

   #173097

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP9 (PARP9 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   N310-N493

   Promoter

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDL-hu FceRI alpha

   Plasmid

   #8365

   Depositor

   Jean Kinet

   Article

   Kochan et al Nucleic Acids Res 1988 Apr 25;16(8):3584

   Insert

   human Fc epsilon RI alpha cDNA (FCER1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pCDNA-3xHA-Reptin

   Plasmid

   #51636

   Purpose

   Mamamlian expression of 3xHA tagged reptin, N-terminal.

   Depositor

   Steven Artandi

   Article

   Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

   Insert

   reptin (RUVBL2 Human)
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-MAP1LC3B

    Plasmid

    #137757

    Purpose

    Expression of LC3B

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK321 (TOP2A-mAC (Neo))

    Plasmid

    #140652

    Purpose

    TOP2A tagging with mAID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-Rab27B N133I

    Plasmid

    #89449

    Purpose

    Expression of GFP-tagged human Rab27B N133I in mammalian cells

    Depositor

    Wendy Westbroek

    Article

    Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

    Insert

    human Rab27B N133I (RAB27B Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    N133I

    Promoter

    CMV

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNAI-GAL4-CREB

    Plasmid

    #46769

    Purpose

    Expression of GAL4 fusion to wild type CREB

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    GAL4(1-147)-CREB (Creb1, GAL4 Budding Yeast, Rat)
    

    Use

    Tags

    GAL4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. HBA1 full 3'UTR-T4 td downstream intron

    Plasmid

    #189974

    Purpose

    Part 6 plasmid to introduce the HBA1 full 3'UTR and T4 td downstream intron into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    HBA1 full 3'UTR-T4 td downstream intron
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pInt

    Plasmid

    #18941

    Depositor

    Michele Calos

    Article

    Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

    Insert

    phiC31 integrase (int Streptomyces phage phiC31)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    under lacZ promoter

    Promoter

    Available since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-mRuby2

    Plasmid

    #40260

    Purpose

    RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Insert

    mRuby2
    

    Use

    Tags

    His6 and Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCE-BiFC-VC155

    Plasmid

    #22020

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Nat Protoc. 2008 . 3(4):588-96.

    Insert

    VC155
    

    Use

    Tags

    HA

    Expression

    Worm

    Mutation

    Changed Alanine 206 to Lysine

    Promoter

    Available since

    Nov. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE

    Plasmid

    #138143

    Purpose

    Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PguCas13b
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-P4M-SidMx2

    Plasmid

    #51472

    Purpose

    Biosensor for PtdIns4P

    Depositor

    Tamas Balla

    Article

    Hammond et al J Cell Biol. 2014 Apr 7.

    Inserts

    P4M-SidM(546-647)
    P4M-SidM(546-647)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSIM24-GUS

    Plasmid

    #67882

    Purpose

    Plant Gene Expression Vector pSIM24 containing the GUS (Beta-glucuronidase) reporter gene

    Depositor

    Indu Maiti

    Article

    Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

    Insert

    uidA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    T120A

    Promoter

    M24

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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