Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19943||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5437
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert nameNEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8
SpeciesH. sapiens (human)
Insert Size (bp)245
Entrez GeneNEDD8 (a.k.a. NEDD-8)
/ Fusion Protein
- myc3 (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
N51S in seq does not appear to affect function
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-myc3-NEDD8 was a gift from Yue Xiong (Addgene plasmid # 19943 ; http://n2t.net/addgene:19943 ; RRID:Addgene_19943)
For your References section:The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1. Furukawa M, Zhang Y, McCarville J, Ohta T, Xiong Y. Mol Cell Biol. 2000 Nov . 20(21):8185-97. 10.1128/MCB.20.21.8185-8197.2000 PubMed 11027288
Map uploaded by the depositor.