|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20753||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 2513
Vector typePCR-based C-terminal tagging
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (unknown if destroyed)
- 3′ cloning site PmeI (unknown if destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFA6a-6xGLY-3xFLAG-HIS3MX6 was a gift from Mark Hochstrasser (Addgene plasmid # 20753 ; http://n2t.net/addgene:20753 ; RRID:Addgene_20753)
For your References section:Small epitope-linker modules for PCR-based C-terminal tagging in Saccharomyces cerevisiae. Funakoshi M, Hochstrasser M. Yeast. 2009 Feb 25. 26(3):185-192. 10.1002/yea.1658 PubMed 19243080