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SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture.
Fierer JO, Veggiani G, Howarth M
Proc Natl Acad Sci U S A. 2014 Mar 17.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
51722SpyLigasePlasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!
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51723SUMO-KTagPlasmid for recombinant expression in bacteria of SUMO domain fused to KTag, permitting covalent linkage to SpyTag
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