Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

pET28a-SrtAdelta59 Citations (6)

Originally described in: Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions.
Guimaraes CP, Witte MD, Theile CS, Bozkurt G, Kundrat L, Blom AE, Ploegh HL Nat Protoc. 2013 Sep;8(9):1787-99. doi: 10.1038/nprot.2013.101. Epub 2013 Aug 29. PubMed Journal

Articles Citing pET28a-SrtAdelta59

Articles
Site-specific protein modification using immobilized sortase in batch and continuous-flow systems. Witte MD, Wu T, Guimaraes CP, Theile CS, Blom AE, Ingram JR, Li Z, Kundrat L, Goldberg SD, Ploegh HL. Nat Protoc. 2015 Mar;10(3):508-16. doi: 10.1038/nprot.2015.026. Epub 2015 Feb 26. PubMed
Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores. Petrovic A, Keller J, Liu Y, Overlack K, John J, Dimitrova YN, Jenni S, van Gerwen S, Stege P, Wohlgemuth S, Rombaut P, Herzog F, Harrison SC, Vetter IR, Musacchio A. Cell. 2016 Nov 3;167(4):1028-1040.e15. doi: 10.1016/j.cell.2016.10.005. Epub 2016 Oct 27. PubMed
ESCORTing proteins directly from whole cell-lysate for single-molecule studies. Srinivasan S, Hazra JP, Singaraju GS, Deb D, Rakshit S. Anal Biochem. 2017 Oct 15;535:35-42. doi: 10.1016/j.ab.2017.07.022. Epub 2017 Jul 26. PubMed
A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging. Virant D, Traenkle B, Maier J, Kaiser PD, Bodenhofer M, Schmees C, Vojnovic I, Pisak-Lukats B, Endesfelder U, Rothbauer U. Nat Commun. 2018 Mar 2;9(1):930. doi: 10.1038/s41467-018-03191-2. PubMed

Associated Plasmids
Force-activated catalytic pathway accelerates bacterial adhesion against flow. Hazra JP, Arora N, Sagar A, Srinivasan S, Chaudhuri A, Rakshit S. Biochem J. 2018 Aug 30;475(16):2611-2620. doi: 10.1042/BCJ20180358. PubMed
Turning antibodies off and on again using a covalently tethered blocking peptide. Brasino M, Wagnell E, Hamilton S, Ranganathan S, Gomes MM, Branchaud B, Messmer B, Ibsen SD. Commun Biol. 2022 Dec 10;5(1):1357. doi: 10.1038/s42003-022-04094-1. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.