|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24317||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4969
Vector typeBacterial Expression
Growth in Bacteria
Gene/Insert namehuman AGO2
SpeciesH. sapiens (human)
Insert Size (bp)2500
Entrez GeneAGO2 (a.k.a. CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI* (destroyed during cloning)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pGEX-5
- 3′ sequencing primer pGEX-3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
*Note that though the depositor lists XhoI as the 5' cloning enzyme, the AGO2 gene has an internal XhoI site. Addgene's quality control sequence shows that there is no intact XhoI site at the 5' end of the insert.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEX-GST-AGO2 was a gift from Carl Novina (Addgene plasmid # 24317 ; http://n2t.net/addgene:24317 ; RRID:Addgene_24317)
For your References section:Distinct passenger strand and mRNA cleavage activities of human Argonaute proteins. Wang B, Li S, Qi HH, Chowdhury D, Shi Y, Novina CD. Nat Struct Mol Biol. 2009 Dec . 16(12):1259-66. 10.1038/nsmb.1712 PubMed 19946268