165,343 results

1. pICH47742:: pRPS5a-PE2max-NC::tHSP

   Plasmid

   #227694

   Purpose

   Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Insert

   PE2max-NC being driven by pRPS5a and terminated by tHSP
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   pRPS5a

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0121

   Plasmid

   #141526

   Purpose

   Lentiviral vector for overexpressing the BARX2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   BARX2 (BARX2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJC175e

   Plasmid

   #79219

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PPSP gIII, luxAB
   

   Expression

   Bacterial

   Promoter

   PPSP

   Available Since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmScarlet-i_C1

   Plasmid

   #85044

   Purpose

   Mammalian expression of mScarlet-i

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Has Service

   Cloning Grade DNA

   Insert

   mScarlet-i
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP

   Plasmid

   #176015

   Purpose

   Mammalian expression of EGFP. Parton lab clone DFS

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   EGFP
   

   Expression

   Mammalian

   Available Since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Human SERCA2a (pcDNA3.1+)

   Plasmid

   #75187

   Purpose

   Mammalian expression of ATP2A2

   Depositor

   David MacLennan, Jonathan Lytton

   Article

   Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

   Insert

   ATP2A2 (ATP2A2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEFIRES-P-ACSL3-mCherry

   Plasmid

   #87158

   Purpose

   Fluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   ACSL3 (ACSL3 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   EF-1-alpha

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3XFlagNICD2

   Plasmid

   #20184

   Depositor

   Raphael Kopan

   Article

   Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

   Insert

   Notch 2 intracellular domain (Notch2 Mouse)
   

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Contains murine Notch 2 Intracellular Domain (Met…

   Promoter

   CMV

   Available Since

   Jan. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE2 Lnp-mCherry

   Plasmid

   #86687

   Purpose

   Lentivirus to stably express fluorescent human protein in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   LNPK (LNPK Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. A*24:02:01

    Plasmid

    #203295

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*24:02:01 (HLA-A Human)
    

    Expression

    Mammalian

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSD080A

    Plasmid

    #218138

    Purpose

    Lentiviral vector expressing evolved degron SD40 fused to eGFP with mCherry control.

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD40-eGFP-IRES2-mCherry
    

    Use

    Lentiviral

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-FLAG-MTOR-E1799K

    Plasmid

    #69009

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-E1799K (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Glutamate 1799 to Lysine

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.CAG.GCaMP6f.WPRE.SV40

    Plasmid

    #100836

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV2, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    CAG

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR223-BORF2

    Plasmid

    #195834

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BORF2

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BORF2
    

    Use

    Gateway entry vector

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-PTEN-C124S

    Plasmid

    #28300

    Depositor

    Maria-Magdalena Georgescu

    Article

    Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

    Insert

    PTEN (PTEN Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Phosphatase Inactive Mutant Cys124Ser

    Available Since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pDONR-221-P3-P4

    Plasmid

    #186351

    Purpose

    Gateway donor vector with AttP3 AttP4 recombination sites

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Type

    Empty backbone

    Use

    Gateway donor vector

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBMN-2xFKBP-mEGFP-NDP52

    Plasmid

    #110465

    Purpose

    retrovirus construct for stable expressing mEGFP tagged NDP52

    Depositor

    Richard Youle

    Article

    Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Retroviral; Retrovirus

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lp-BFPnls-LP-Cherry-T2A-NTR2.0

    Plasmid

    #219785

    Purpose

    Used for generating a transgenic line for inducing NTR2.0 expression in Cre positive cells after conversion

    Depositor

    Prayag Murawala

    Article

    García-García et al bioRxiv 2025.03.30.645595; doi: 10.1101/

    Insert

    mTagBFP2
    

    Tags

    3x SV40 NLS

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. dCAS9-VP64_GFP

    Plasmid

    #61422

    Purpose

    Expresses dCAS9-VP64 activator with 2A GFP

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Concentrated Lentiviral Prep

    Insert

    dCAS9(D10A,H840A)-VP64_2A_GFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    EF1A

    Available Since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-15b-EK_C122S_His5

    Plasmid

    #49048

    Purpose

    Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

    Depositor

    Hans Brandstetter

    Article

    Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

    Insert

    Enteropeptidase, peptidase domain (TMPRSS15 Bovine)
    

    Tags

    5xHis

    Expression

    Bacterial

    Mutation

    P92R, E185D, C122S

    Promoter

    T7

    Available Since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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