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Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors.
Tak YE, Kleinstiver BP, Nunez JK, Hsu JY, Horng JE, Gong J, Weissman JS, Joung JK
Nat Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
104563MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHAMammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)
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104564BPK1169: CAG-DmrC-NLS-3xFLAG-P65Mammalian expression vector for DmrC fused to p65 activation domain
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104565MMW948: CAG-DmrC-NLS-3xFLAG-VPRMammalian expression vector for DmrC fused to VPR activator
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104566JG1202: CAG-human dLbCpf1(D832A)-NLS-3xHA-P65Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to p65 activation domain
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104567JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPRMammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator
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104568JG674: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X1)Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to one DmrA domain
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104569JG676: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X2)Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to two DmrA domains
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104570JG693: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X3)Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to three DmrA domains
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104571YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains
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