PurposeMammalian expression vector for DmrC fused to p65 activation domain
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||104564||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerCepko lab (Addgene plasmid # 11179)
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt nameDmrC domain fused to NF-KB p65 activation domain
- Promoter CAG
/ Fusion Proteins
- NLS (C terminal on insert)
- 3x FLAG (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
- 3′ sequencing primer oBK23-R (5'-CAGAGGGAAAAAGATCTCAGTGG-3') (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:BPK1169: CAG-DmrC-NLS-3xFLAG-P65 was a gift from Keith Joung (Addgene plasmid # 104564 ; http://n2t.net/addgene:104564 ; RRID:Addgene_104564)
For your References section:Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors. Tak YE, Kleinstiver BP, Nunez JK, Hsu JY, Horng JE, Gong J, Weissman JS, Joung JK. Nat Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483. 10.1038/nmeth.4483 PubMed 29083402