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MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA
(Plasmid #104563)


Item Catalog # Description Quantity Price (USD)
Plasmid 104563 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Cepko lab (Addgene plasmid # 11179)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    dLbCpf1 (D832A)
  • Alt name
    human codon optimized ‘dead’ Cpf1
  • Species
    Lachnospiraceae bacterium
  • Mutation
  • Promoter CAG
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • 3x HA (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
  • 3′ sequencing primer oBK23-R (5'-CAGAGGGAAAAAGATCTCAGTGG-3')
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA was a gift from Keith Joung (Addgene plasmid # 104563 ; ; RRID:Addgene_104563)
  • For your References section:

    Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors. Tak YE, Kleinstiver BP, Nunez JK, Hsu JY, Horng JE, Gong J, Weissman JS, Joung JK. Nat Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483. 10.1038/nmeth.4483 PubMed 29083402