PurposeGFP-tagged BRD protein, which can be used to be expressed in mammaiian cells.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||65377||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5692
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2178
Entrez GeneBRD3 (a.k.a. ORFX, RING3L)
- Promoter CMV
/ Fusion Proteins
- EmGFP (N terminal on backbone)
- V5 (C terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer EGFP_C_primer
- 3′ sequencing primer TK polyA reverse primer (Common Sequencing Primers)
Gene Source: DNASU HsCD0022366
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GFP-BRD3 was a gift from Kyle Miller (Addgene plasmid # 65377 ; http://n2t.net/addgene:65377 ; RRID:Addgene_65377)
For your References section:Screen identifies bromodomain protein ZMYND8 in chromatin recognition of transcription-associated DNA damage that promotes homologous recombination. Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M, Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM. Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114. 10.1101/gad.252189.114 PubMed 25593309