Purpose(Empty Backbone) Express gene of interest under PGK (phosphoglycerate kinase I) promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||72268||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6396
Modifications to backboneClaI-SalI fragment was replaced with PGK promoter
Vector typeMammalian Expression, Lentiviral
- Promoter PGK
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer AGTGAACGGATCTCGACGGT
- 3′ sequencing primer CAACACCACGGAATTGTCAG (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCDH-PGK was a gift from Kazuhiro Oka (Addgene plasmid # 72268 ; http://n2t.net/addgene:72268 ; RRID:Addgene_72268)