163,606 results

1. FKBP1AA-c001

   Plasmid

   #53671

   Purpose

   bacterial expression plasmid

   Depositor

   Nicola Burgess-Brown

   Insert

   FKBP1AA
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pQCXIX-Myc-hTIM3

   Plasmid

   #110893

   Purpose

   Expression of Myc-tagged hTIM3 at the cell surface

   Depositor

   Hyeryun Choe

   Article

   Jemielity et al PLoS Pathog. 2013 Mar;9(3):e1003232. doi

   Insert

   Hepatitis A virus Cellular Receptor 2 (HAVCR2) (HAVCR2 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. mTagRFP-T-Tubulin-6

   Plasmid

   #58026

   Purpose

   Localization: Microtubules, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Tags

   mTagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pHAGE-EGFR-E746_T751delinsA

   Plasmid

   #116246

   Purpose

   Lentiviral expression of EGFR E746_T751delinsA

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   EGFR (EGFR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   E746_T751delinsA

   Promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUCmini-iCAP-AAV.MaCPNS2

   Plasmid

   #185137

   Purpose

   non-standard AAV2 rep-AAV-MaCPNS2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

   Insert

   Synthetic construct isolate AAV-MaCPNS2 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available Since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Brg1 Del2 pET28a

   Plasmid

   #122116

   Purpose

   Expression Brg1 amino acids 1-837 in bacteria

   Depositor

   Paul Herring

   Article

   Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

   Insert

   Brg1 (SMARCA4 Human)
   

   Use

   Tags

   6XHis and His-T7

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-PIK3C2A

   Plasmid

   #161988

   Purpose

   Mammalian expression vector containing PIK3C2A tagged with GFP

   Depositor

   David Bryant, Volker Haucke

   Article

   Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

   Insert

   PIK3C2A (PIK3C2A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET3a-hUb

   Plasmid

   #61937

   Purpose

   Expression of hUb in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Insert

   human ubiquitin (UBC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pMY-mCherry-P2A-PuroR-HA

   Plasmid

   #163342

   Purpose

   Retroviral vector to express cytosolic mCherry and an HA-tagged puromycin selection marker

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Insert

   mCherry
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR

    Plasmid

    #129774

    Purpose

    Marker of microtubule dynamics in C. elegans hypodermis

    Depositor

    Andrew Chisholm

    Article

    Chuang et al Elife. 2016 Sep 23;5. doi: 10.7554/eLife

    Inserts

    Pcol-19
    ebp-2 (ebp-2 Nematode)
    unc-54 3'UTR
    

    Use

    Synthetic Biology; Gateway

    Tags

    Synthetic Intron and mGFP

    Expression

    Worm

    Mutation

    Promoter

    Pcol-19

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmTurquoise2-Mito

    Plasmid

    #36208

    Purpose

    In vivo visualization of the mitochondria (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    MTS (COX8A Human)
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. SF-U7snRNA-CypA-E4

    Plasmid

    #190695

    Purpose

    Control vector expressing a U7-based antisense oligonucleotide targeting CypA exon 4

    Depositor

    Pim Pijnappel

    Article

    van der Wal et al Mol Ther Nucleic Acids. 2017 Jun 16;7:90

    Insert

    U7 snRNA antisense CypA Exon 4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U7 promoter

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Brg1 Del3 pET28a

    Plasmid

    #122117

    Purpose

    Expression Brg1 amino acids 836-1446 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Use

    Tags

    His-T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. AV15_pCAG.Cas9.gRNA.S1

    Plasmid

    #199259

    Purpose

    Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Inserts

    SpCas9 nuclease
    AAVS1-targeting gRNA
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter and RNA polymerase III promoter for …

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRIS

    Plasmid

    #120424

    Purpose

    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.

    Depositor

    Tamás Fehér

    Article

    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

    Insert

    CRISPR spacers targeting IS1, IS5, IS3, IS150
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    constitutive

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP

    Plasmid

    #176279

    Purpose

    Viral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    Kir2.1MUT-P2A-EGFP (Kcnj2 Synthetic, Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    GYG to AAA (aa144-146)

    Promoter

    human Synapsin I

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Terminase_PhiV1

    Plasmid

    #188836

    Purpose

    Large terminase from phage PhiV1

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    Terminase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLJC6-NADK-3xFLAG

    Plasmid

    #163452

    Purpose

    lentiviral overexpression vector for human NADK

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    NADK (NADK Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    See: comments

    Promoter

    Ubc

    Available Since

    March 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221-TMEM165

    Plasmid

    #132272

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    TMEM165 (TMEM165 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. EGFP-LC3

    Plasmid

    #11546

    Depositor

    Karla Kirkegaard

    Article

    Jackson et al PLoS Biol. 2005 May . 3(5):e156.

    Insert

    LC3B (MAP1LC3B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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